7vwt
From Proteopedia
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==Carbazole Prenyl Transferase CqsB4== | ==Carbazole Prenyl Transferase CqsB4== | ||
| - | <StructureSection load='7vwt' size='340' side='right'caption='[[7vwt]]' scene=''> | + | <StructureSection load='7vwt' size='340' side='right'caption='[[7vwt]], [[Resolution|resolution]] 1.73Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VWT FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7vwt]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VWT FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vwt OCA], [https://pdbe.org/7vwt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vwt RCSB], [https://www.ebi.ac.uk/pdbsum/7vwt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vwt ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AO6:2-methyl-1-[(2R)-2-oxidanylpropyl]-9H-carbazole-3,4-dione'>AO6</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vwt OCA], [https://pdbe.org/7vwt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vwt RCSB], [https://www.ebi.ac.uk/pdbsum/7vwt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vwt ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Some enzymes annotated as squalene synthase catalyze the prenylation of carbazole-3,4-quinone-containing substrates in bacterial secondary metabolism. Their reaction mechanisms remain unclear because of their low sequence similarity to well-characterized aromatic substrate prenyltransferases (PTs). We determined the crystal structures of the carbazole PTs, and these revealed that the overall structure is well superposed on those of squalene synthases. In contrast, the stacking interaction between the prenyl donor and acceptor substrates resembles those observed in aromatic substrate PTs. Structural and mutational analyses suggest that the Ile and Asp residues are essential for the hydrophobic and hydrophilic interactions with the carbazole-3,4-quinone moiety of the prenyl acceptor, respectively, and a deprotonation mechanism of an intermediary sigma-complex involving a catalytic triad is proposed. Our results provide a structural basis for a new subclass of aromatic substrate PTs. | ||
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| + | Structural Basis for the Prenylation Reaction of Carbazole-Containing Natural Products Catalyzed by Squalene Synthase-Like Enzymes.,Nagata R, Suemune H, Kobayashi M, Shinada T, Shin-Ya K, Nishiyama M, Hino T, Sato Y, Kuzuyama T, Nagano S Angew Chem Int Ed Engl. 2022 Mar 2:e202117430. doi: 10.1002/anie.202117430. PMID:35235232<ref>PMID:35235232</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7vwt" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Kuzuyama T]] | + | [[Category: Kuzuyama, T]] |
| - | [[Category: Nagano S]] | + | [[Category: Nagano, S]] |
| - | [[Category: Nagata R]] | + | [[Category: Nagata, R]] |
| - | [[Category: Suemune H]] | + | [[Category: Suemune, H]] |
| + | [[Category: Prenyl transferase]] | ||
| + | [[Category: Squalene synthase]] | ||
| + | [[Category: Transferase]] | ||
Revision as of 11:19, 30 March 2022
Carbazole Prenyl Transferase CqsB4
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