Sandbox Reserved 1721

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== Relevance ==
== Relevance ==
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== Structural highlights ==
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== Structure ==
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===Open Conformation===
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Neurofibromin is a large protein of 2818 amino acids <ref>DOI 10.3390/cells9112365</ref> and is a homodimer that exists in two conformations.
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<scene name='90/904326/Open_conformation/2'>Open Conformation</scene>,
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===Domains===
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<scene name='90/904326/Open_conformation_with_grd_hig/2'>Open Conformation with GRD highlighted</scene>
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N-heat and c-heat
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===Closed Conformation===
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grd
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<scene name='90/904326/Closed_conformation/2'>Closed Conformation</scene>,
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sec14ph
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<scene name='90/904326/Grd_closed_conformation/2'>Closed Conformation with GRD highlighted</scene>
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===conformations===
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===Sec14-Ph Domain===
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====closed====
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<scene name='90/904326/Sec15ph_and_grd_open/2'>sec14ph domain in Open Conformation</scene>
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The closed state of neurofibromin has both protomers in a closed conformation, which inhibits the binding of Ras to the GRD of neurofibromin due to the HEAT/ARM blocking the GRD. A metal binding site between the N-HEAT/ARM domain and the GRD-Sec14-PH linker stabilize the closed conformation. This site is coordinated by three residues, C1032, H1558, and H1576, and a water molecule. This binding site is preferential for zinc.
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<scene name='90/904326/Sec14ph_and_grd_closed/2'>sec14ph domain in Closed Conformation</scene>
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====open====
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===Active Site===
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The open state of neurofibromin has one protomer in a open conformation and the other in a closed conformation. The protomer in the open conformation allows for the binding of Ras because of reorientation of the GRD and Sec14-PH domains. In the open conformation, the metal binding site found in the closed conformation is lost due to separation of the N-HEAT/ARM and the cysteine residue from the histidine residues founds in the GRD-Sec14-PH linker.
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<scene name='90/904326/Active_site_with_residues/6'>active site</scene>
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====transition between open and closed conformation====
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rearrangement of connective loops between domains
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triad of residues from nf
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====Spred1====
</StructureSection>
</StructureSection>
== References ==
== References ==
<references/>
<references/>

Revision as of 19:46, 7 April 2022

This Sandbox is Reserved from February 28 through September 1, 2022 for use in the course CH462 Biochemistry II taught by R. Jeremy Johnson at the Butler University, Indianapolis, USA. This reservation includes Sandbox Reserved 1700 through Sandbox Reserved 1729.
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References

  1. ↑ Hanson, R. M., Prilusky, J., Renjian, Z., Nakane, T. and Sussman, J. L. (2013), JSmol and the Next-Generation Web-Based Representation of 3D Molecular Structure as Applied to Proteopedia. Isr. J. Chem., 53:207-216. doi:http://dx.doi.org/10.1002/ijch.201300024
  2. ↑ Herraez A. Biomolecules in the computer: Jmol to the rescue. Biochem Mol Biol Educ. 2006 Jul;34(4):255-61. doi: 10.1002/bmb.2006.494034042644. PMID:21638687 doi:10.1002/bmb.2006.494034042644
  3. ↑ Bergoug M, Doudeau M, Godin F, Mosrin C, Vallee B, Benedetti H. Neurofibromin Structure, Functions and Regulation. Cells. 2020 Oct 27;9(11). pii: cells9112365. doi: 10.3390/cells9112365. PMID:33121128 doi:http://dx.doi.org/10.3390/cells9112365
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