6tav
From Proteopedia
(Difference between revisions)
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==Crystal structure of endopeptidase-induced alpha2-macroglobulin== | ==Crystal structure of endopeptidase-induced alpha2-macroglobulin== | ||
| - | <StructureSection load='6tav' size='340' side='right'caption='[[6tav]]' scene=''> | + | <StructureSection load='6tav' size='340' side='right'caption='[[6tav]], [[Resolution|resolution]] 4.20Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TAV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TAV FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6tav]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4acq 4acq]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TAV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TAV FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tav OCA], [https://pdbe.org/6tav PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tav RCSB], [https://www.ebi.ac.uk/pdbsum/6tav PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tav ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">A2M, CPAMD5, FWP007 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tav OCA], [https://pdbe.org/6tav PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tav RCSB], [https://www.ebi.ac.uk/pdbsum/6tav PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tav ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/A2MG_HUMAN A2MG_HUMAN]] Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | I'm your Venus: The crystal structure of the human methylamine-induced form of alpha(2) -macroglobulin (alpha(2) M) shows its large central cavity can accommodate two medium-sized proteinases (see structure, front part clipped off to better show central cavity). Twelve major entrances provide access for small substrates to the cavity and the still-active trapped "prey". The structure unveils the molecular basis of the unique "venus flytrap" mechanism of alpha(2) M. | ||
| + | |||
| + | The Crystal Structure of Human alpha(2) -Macroglobulin Reveals a Unique Molecular Cage.,Marrero A, Duquerroy S, Trapani S, Goulas T, Guevara T, Andersen GR, Navaza J, Sottrup-Jensen L, Gomis-Ruth FX Angew Chem Int Ed Engl. 2012 Jan 31. doi: 10.1002/anie.201108015. PMID:22290936<ref>PMID:22290936</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6tav" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Human]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Andersen | + | [[Category: Andersen, G A]] |
| - | [[Category: Duquerroy S]] | + | [[Category: Duquerroy, S]] |
| - | [[Category: Gomis-Ruth | + | [[Category: Gomis-Ruth, F X]] |
| - | [[Category: Goulas T]] | + | [[Category: Goulas, T]] |
| - | [[Category: Guevara T]] | + | [[Category: Guevara, T]] |
| - | [[Category: Marrero A]] | + | [[Category: Marrero, A]] |
| - | [[Category: Navaza J]] | + | [[Category: Navaza, J]] |
| - | [[Category: Sottrup-Jensen L]] | + | [[Category: Sottrup-Jensen, L]] |
| - | [[Category: Trapani S]] | + | [[Category: Trapani, S]] |
| + | [[Category: Cytokine binding]] | ||
| + | [[Category: Hormone binding]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Peptidase inhibitor]] | ||
| + | [[Category: Protein binding]] | ||
| + | [[Category: Proteinase inhibitor]] | ||
| + | [[Category: Zn-and cu-binding protein]] | ||
Revision as of 09:43, 13 April 2022
Crystal structure of endopeptidase-induced alpha2-macroglobulin
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Categories: Human | Large Structures | Andersen, G A | Duquerroy, S | Gomis-Ruth, F X | Goulas, T | Guevara, T | Marrero, A | Navaza, J | Sottrup-Jensen, L | Trapani, S | Cytokine binding | Hormone binding | Hydrolase | Peptidase inhibitor | Protein binding | Proteinase inhibitor | Zn-and cu-binding protein
