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2xlj

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Revision as of 10:55, 13 April 2022

Crystal structure of the Csy4-crRNA complex, hexagonal form

Structural highlights

2xlj is a 2 chain structure with sequence from Pseab. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	2xli, 2xlk
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Many bacteria and archaea contain clustered regularly interspaced short palindromic repeats (CRISPRs) that confer resistance to invasive genetic elements. Central to this immune system is the production of CRISPR-derived RNAs (crRNAs) after transcription of the CRISPR locus. Here, we identify the endoribonuclease (Csy4) responsible for CRISPR transcript (pre-crRNA) processing in Pseudomonas aeruginosa. A 1.8 angstrom crystal structure of Csy4 bound to its cognate RNA reveals that Csy4 makes sequence-specific interactions in the major groove of the crRNA repeat stem-loop. Together with electrostatic contacts to the phosphate backbone, these enable Csy4 to bind selectively and cleave pre-crRNAs using phylogenetically conserved serine and histidine residues in the active site. The RNA recognition mechanism identified here explains sequence- and structure-specific processing by a large family of CRISPR-specific endoribonucleases.

Sequence- and structure-specific RNA processing by a CRISPR endonuclease.,Haurwitz RE, Jinek M, Wiedenheft B, Zhou K, Doudna JA Science. 2010 Sep 10;329(5997):1355-8. PMID:20829488^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Haurwitz RE, Jinek M, Wiedenheft B, Zhou K, Doudna JA. Sequence- and structure-specific RNA processing by a CRISPR endonuclease. Science. 2010 Sep 10;329(5997):1355-8. PMID:20829488 doi:10.1126/science.1192272

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2xlj"

@@ Line 3: / Line 3: @@
 <StructureSection load='2xlj' size='340' side='right'caption='[[2xlj]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2xlj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseab Pseab]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XLJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2XLJ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2xlj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseab Pseab]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XLJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XLJ FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2xli|2xli]], [[2xlk|2xlk]]</td></tr>
+</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2xli|2xli]], [[2xlk|2xlk]]</div></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2xlj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xlj OCA], [http://pdbe.org/2xlj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xlj RCSB], [http://www.ebi.ac.uk/pdbsum/2xlj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xlj ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xlj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xlj OCA], [https://pdbe.org/2xlj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xlj RCSB], [https://www.ebi.ac.uk/pdbsum/2xlj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xlj ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==

2xlj

From Proteopedia

Revision as of 10:55, 13 April 2022

Crystal structure of the Csy4-crRNA complex, hexagonal form

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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