3i58
From Proteopedia
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==Crystal structure of an O-methyltransferase (NcsB1) from neocarzinostatin biosynthesis in complex with S-adenosyl-L-homocysteine (SAH) and 2-hydroxy-7-methoxy-5-methyl naphthoic acid (NA)== | ==Crystal structure of an O-methyltransferase (NcsB1) from neocarzinostatin biosynthesis in complex with S-adenosyl-L-homocysteine (SAH) and 2-hydroxy-7-methoxy-5-methyl naphthoic acid (NA)== | ||
| - | <StructureSection load='3i58' size='340' side='right' caption='[[3i58]], [[Resolution|resolution]] 2.69Å' scene=''> | + | <StructureSection load='3i58' size='340' side='right'caption='[[3i58]], [[Resolution|resolution]] 2.69Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[3i58]] is a 2 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[3i58]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"streptomyces_carzinostaticus_subsp._neocarzinostaticus"_ishida_et_al. "streptomyces carzinostaticus subsp. neocarzinostaticus" ishida et al.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I58 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I58 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7NA:2-HYDROXY-7-METHOXY-5-METHYL+NAPHTHOIC+ACID'>7NA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7NA:2-HYDROXY-7-METHOXY-5-METHYL+NAPHTHOIC+ACID'>7NA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3i53|3i53]], [[3i5u|3i5u]], [[3i64|3i64]]</td></tr> | + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3i53|3i53]], [[3i5u|3i5u]], [[3i64|3i64]]</div></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ncsb1 ([ | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ncsb1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=167636 "Streptomyces carzinostaticus subsp. neocarzinostaticus" Ishida et al.])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i58 OCA], [https://pdbe.org/3i58 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i58 RCSB], [https://www.ebi.ac.uk/pdbsum/3i58 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i58 ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [[ | + | [[https://www.uniprot.org/uniprot/NCSB1_STRCZ NCSB1_STRCZ]] S-adenosyl-L-methionine-dependent O-methyltransferase that catalyzes regiospecific methylation at the 7-hydroxy group of 2,7-dihydroxy-5-methyl-1-naphthoate in the biosynthesis of the naphthoate moiety of the neocarzinostatin chromophore. Also recognizes other dihydroxynaphthoate as substrates and catalyzes their regiospecific O-methylation. The carboxylate and its ortho-hydroxy groups of the substrate appear to be crucial for NcsB1 substrate recognition and binding, and O-methylation takes place only at the free hydroxy group of these dihydroxynaphthoic acids.<ref>PMID:18387946</ref> |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Streptomyces carzinostaticus subsp. neocarzinostaticus ishida et al]] | [[Category: Streptomyces carzinostaticus subsp. neocarzinostaticus ishida et al]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Bruner, S D]] | [[Category: Bruner, S D]] | ||
[[Category: Cooke, H A]] | [[Category: Cooke, H A]] | ||
Revision as of 10:57, 13 April 2022
Crystal structure of an O-methyltransferase (NcsB1) from neocarzinostatin biosynthesis in complex with S-adenosyl-L-homocysteine (SAH) and 2-hydroxy-7-methoxy-5-methyl naphthoic acid (NA)
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