1gw0

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[[Image:1gw0.jpg|left|200px]]
[[Image:1gw0.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1gw0 |SIZE=350|CAPTION= <scene name='initialview01'>1gw0</scene>, resolution 2.4&Aring;
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The line below this paragraph, containing "STRUCTURE_1gw0", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+B'>AC1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1gw0| PDB=1gw0 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gw0 OCA], [http://www.ebi.ac.uk/pdbsum/1gw0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1gw0 RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF LACCASE FROM MELANOCARPUS ALBOMYCES IN FOUR COPPER FORM'''
'''CRYSTAL STRUCTURE OF LACCASE FROM MELANOCARPUS ALBOMYCES IN FOUR COPPER FORM'''
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[[Category: Rouvinen, J.]]
[[Category: Rouvinen, J.]]
[[Category: Saloheimo, M.]]
[[Category: Saloheimo, M.]]
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[[Category: ascomycete]]
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[[Category: Ascomycete]]
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[[Category: c-terminal plug]]
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[[Category: C-terminal plug]]
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[[Category: laccase]]
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[[Category: Laccase]]
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[[Category: multi-copper oxidase]]
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[[Category: Multi-copper oxidase]]
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[[Category: oxygen reduction]]
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[[Category: Oxygen reduction]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:04:45 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:52:32 2008''
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Revision as of 15:04, 2 May 2008

Image:1gw0.jpg

Template:STRUCTURE 1gw0

CRYSTAL STRUCTURE OF LACCASE FROM MELANOCARPUS ALBOMYCES IN FOUR COPPER FORM


Overview

We have crystallized the ascomycete laccase from Melanocarpus albomyces with all four coppers present and determined the crystal structure at 2.4 A resolution. The enzyme is heavily glycosylated and consists of three cupredoxin-like domains, similar to those found in the Cu-depleted basidiomycete laccase from Coprinus cinereus. However, there are significant differences in the loops forming the substrate-binding pocket. In addition, the crystal structure of the M. albomyces laccase revealed elongated electron density between all three coppers in the trinuclear copper site, suggesting that an oxygen molecule binds with a novel geometry. This oxygen, required in the reaction, may enter the trinuclear site through the tunnel, which is open in the structure of the C. cinereus laccase. In contrast, the C-terminus on the M. albomyces laccase forms a plug that blocks this access.

About this Structure

1GW0 is a Single protein structure of sequence from Melanocarpus albomyces. Full crystallographic information is available from OCA.

Reference

Crystal structure of a laccase from Melanocarpus albomyces with an intact trinuclear copper site., Hakulinen N, Kiiskinen LL, Kruus K, Saloheimo M, Paananen A, Koivula A, Rouvinen J, Nat Struct Biol. 2002 Aug;9(8):601-5. PMID:12118243 Page seeded by OCA on Fri May 2 18:04:45 2008

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