1ei0
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(New page: 200px<br /> <applet load="1ei0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ei0" /> '''NMR STRUCTURE OF THE ALPHA-HELICAL HAIRPIN ...)
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Revision as of 14:37, 12 November 2007
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NMR STRUCTURE OF THE ALPHA-HELICAL HAIRPIN OF P8MTCP1
Overview
Helical coiled-coils and bundles are some of the most common structural, motifs found in proteins. Design and synthesis of alpha-helical motifs may, provide interesting scaffolds that can be useful as host structures to, display functional sites, thus allowing the engineering of novel, functional miniproteins. We have synthesized a 38-amino acid peptide, alpha2p8, encompassing the alpha-helical hairpin present in the structure, of p8MTCP1, as an alpha-helical scaffold particularly promising for its, stability and permissiveness of sequence mutations. The three-dimensional, structure of this peptide has been solved using homonuclear, two-dimensional NMR techniques at 600 MHz. After sequence specific, assignment, a total of 285 distance and 29 dihedral restraints were, collected. The solution structure of alpha2p8 is presented as a set of 30, DIANA structures, further refined by restrained molecular dynamics, using, simulated annealing protocol with the AMBER force field. The RMSD values, for the backbone and all heavy atoms are 0.65+/-0.25 and 1.51+/-0.21 A, respectively. Excised from its protein context, the alpha-hairpin keeps, its native structure: an alpha-helical coiled-coil, similar to that found, in superhelical structures, with two helices spanning residues 4-16 and, 25-36, and linked by a short loop. This motif is stabilized by two, interhelical disulfide bridges and several hydrophobic interactions at the, helix interface, leaving most of its solvent-exposed surface available for, mutation. This alpha-helical hairpin, easily amenable to synthetic, chemistry and biological expression system, may represent a stable and, versatile scaffold to display new functional sites and peptide libraries.
About this Structure
1EI0 is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Synthesis and NMR solution structure of an alpha-helical hairpin stapled with two disulfide bridges., Barthe P, Rochette S, Vita C, Roumestand C, Protein Sci. 2000 May;9(5):942-55. PMID:10850804
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