1gxw
From Proteopedia
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'''THE 2.2 A RESOLUTION STRUCTURE OF THERMOLYSIN CRYSTALLIZED IN PRESENCE OF POTASSIUM THIOCYANATE''' | '''THE 2.2 A RESOLUTION STRUCTURE OF THERMOLYSIN CRYSTALLIZED IN PRESENCE OF POTASSIUM THIOCYANATE''' | ||
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[[Category: Selkti, M.]] | [[Category: Selkti, M.]] | ||
[[Category: Tomas, A.]] | [[Category: Tomas, A.]] | ||
| - | [[Category: | + | [[Category: Hydrolase]] |
| - | [[Category: | + | [[Category: Metalloendopeptidase]] |
| - | [[Category: | + | [[Category: Metalloprotease]] |
| - | [[Category: | + | [[Category: Salting-in]] |
| - | [[Category: | + | [[Category: Thermolysin]] |
| - | [[Category: | + | [[Category: Thiocyanate]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:09:32 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 15:09, 2 May 2008
THE 2.2 A RESOLUTION STRUCTURE OF THERMOLYSIN CRYSTALLIZED IN PRESENCE OF POTASSIUM THIOCYANATE
Overview
A new crystallization protocol for thermolysin (EC 3.4.24.27) from Bacillus thermoproteolyticus is presented. After dissolving the protein in the presence of KSCN, which avoids the use of DMSO and CsCl, crystals were obtained following the salting-in method. Crystal cell parameters are isomorphous with those previously reported from DMSO/CsCl mixtures. The new SCN(-) crystal structure has been analyzed. It shows the presence of one thiocyanate ion in the catalytic site and several rearrangements in the S(1) and S(2) subsites. These results are in agreement with the measurements of Inouye et al. [(1998), J. Biochem. (Tokyo), 123, 847-852], who observed in solution that the solubility of TLN, which is particularly poor in low ionic strength solutions, increases dramatically in the presence of several neutral salts. The results reported here suggest possible explanations for the solubility increase and for the inhibitory effects of high SCN(-) concentrations on thermolysin activity.
About this Structure
1GXW is a Single protein structure of sequence from Bacillus thermoproteolyticus. Full crystallographic information is available from OCA.
Reference
The 2.2 A resolution structure of thermolysin (TLN) crystallized in the presence of potassium thiocyanate., Gaucher JF, Selkti M, Prange T, Tomas A, Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2198-200. Epub, 2002 Nov 23. PMID:12454500 Page seeded by OCA on Fri May 2 18:09:32 2008
