7beu
From Proteopedia
(Difference between revisions)
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==Human glutathione transferase M1-1== | ==Human glutathione transferase M1-1== | ||
| - | <StructureSection load='7beu' size='340' side='right'caption='[[7beu]]' scene=''> | + | <StructureSection load='7beu' size='340' side='right'caption='[[7beu]], [[Resolution|resolution]] 1.59Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BEU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BEU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7beu]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BEU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BEU FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7beu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7beu OCA], [https://pdbe.org/7beu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7beu RCSB], [https://www.ebi.ac.uk/pdbsum/7beu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7beu ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7beu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7beu OCA], [https://pdbe.org/7beu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7beu RCSB], [https://www.ebi.ac.uk/pdbsum/7beu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7beu ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/GSTM1_HUMAN GSTM1_HUMAN]] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.<ref>PMID:16548513</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Glutathione transferases (GSTs; EC 2.5.1.18) form a group of multifunctional enzymes that are involved in phase II of the cellular detoxification mechanism and are associated with increased susceptibility to cancer development and resistance to anticancer drugs. The present study aims to evaluate the ligandability of the human GSTM1-1 isoenzyme (hGSTM1-1) using a broad range of structurally diverse pesticides as probes. The results revealed that hGSTM1-1, compared to other classes of GSTs, displays limited ligandability and ligand-binding promiscuity, as revealed by kinetic inhibition studies. Among all tested pesticides, the carbamate insecticide pirimicarb was identified as the strongest inhibitor towards hGSTM1-1. Kinetic inhibition analysis showed that pirimicarb behaved as a mixed-type inhibitor toward glutathione (GSH) and 1-chloro-2,4-dinitrobenzene (CDNB). To shine a light on the restricted hGSTM1-1 ligand-binding promiscuity, the ligand-free crystal structure of hGSTM1-1 was determined by X-ray crystallography at 1.59 A-resolution. Comparative analysis of ligand-free structure with the available ligand-bound structures allowed for the study of the enzyme's plasticity and the induced-fit mechanism operated by hGSTM1-1. The results revealed important structural features of the H-site that contribute to xenobiotic-ligand binding and specificity. It was concluded that hGSTM1-1 interacts preferentially with one-ring aromatic compounds that bind at a discrete site which partially overlaps with the xenobiotic substrate binding site (H-site). The results of the study form a basis for the rational design of new drugs targeting hGSTM1-1. | ||
| + | |||
| + | Ligandability Assessment of Human Glutathione Transferase M1-1 Using Pesticides as Chemical Probes.,Bodourian CS, Poudel N, Papageorgiou AC, Antoniadi M, Georgakis ND, Abe H, Labrou NE Int J Mol Sci. 2022 Mar 25;23(7). pii: ijms23073606. doi: 10.3390/ijms23073606. PMID:35408962<ref>PMID:35408962</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7beu" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Glutathione transferase]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Papageorgiou | + | [[Category: Papageorgiou, A C]] |
| - | [[Category: Poudel N]] | + | [[Category: Poudel, N]] |
| + | [[Category: Detoxification]] | ||
| + | [[Category: Glutathione]] | ||
| + | [[Category: Ligand binding]] | ||
| + | [[Category: Transferase]] | ||
| + | [[Category: Xenobiotic]] | ||
Revision as of 11:46, 27 April 2022
Human glutathione transferase M1-1
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