7o4x
From Proteopedia
(Difference between revisions)
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==Crystal structure of the PII-like protein PotN from Lentilactobacillus hilgardii== | ==Crystal structure of the PII-like protein PotN from Lentilactobacillus hilgardii== | ||
| - | <StructureSection load='7o4x' size='340' side='right'caption='[[7o4x]]' scene=''> | + | <StructureSection load='7o4x' size='340' side='right'caption='[[7o4x]], [[Resolution|resolution]] 1.65Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O4X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O4X FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7o4x]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O4X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O4X FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o4x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o4x OCA], [https://pdbe.org/7o4x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o4x RCSB], [https://www.ebi.ac.uk/pdbsum/7o4x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o4x ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o4x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o4x OCA], [https://pdbe.org/7o4x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o4x RCSB], [https://www.ebi.ac.uk/pdbsum/7o4x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o4x ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | PII proteins are signal processor proteins that regulate the cellular metabolism of Bacteria, Archea and plant chloroplasts typically in response to the cellular nitrogen status. Here, we report the first biochemical characterization of a novel PII-like protein PotN from Lentilactobacillus hilgardii. PotN is encoded in an operon together with the potABCD genes, encoding the ABC transporter for spermidine/putrescine. Like canonical PII proteins, the native PotN has a trimeric structure and competitively binds ATP and ADP, but it does not bind 2-oxoglutarate. Immunoprecipitation and pull-down experiments revealed that PotN is associated in vivo with the transcriptional regulator GlnR and the beta-subunit of pyruvate/2-oxoglutarate/acetoin dehydrogenase AcoB. Moreover, in vitro assays revealed that the ATPase domain of PotA also is able to interact with PotN. Interaction analyses demonstrated that PotN preferentially associates with PotA in the ADP state, whereas it binds to GlnR at elevated ATP levels. This suggests that PotN regulates the transport of polyamines and GlnR-dependent gene expression in response to the energy availability for the cell. | ||
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| + | PotN represents a novel energy-state sensing PII subfamily, occurring in firmicutes.,Iskhakova ZI, Zhuravleva DE, Heim C, Hartmann MD, Laykov AV, Forchhammer K, Kayumov AR FEBS J. 2022 Mar 14. doi: 10.1111/febs.16431. PMID:35285159<ref>PMID:35285159</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7o4x" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Hartmann | + | [[Category: Hartmann, M D]] |
| - | [[Category: Heim C]] | + | [[Category: Heim, C]] |
| + | [[Category: Signaling protein]] | ||
Revision as of 11:50, 27 April 2022
Crystal structure of the PII-like protein PotN from Lentilactobacillus hilgardii
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