7wbu
From Proteopedia
(Difference between revisions)
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==Cryo-EM structure of bovine NLRP9== | ==Cryo-EM structure of bovine NLRP9== | ||
| - | <StructureSection load='7wbu' size='340' side='right'caption='[[7wbu]]' scene=''> | + | <StructureSection load='7wbu' size='340' side='right'caption='[[7wbu]], [[Resolution|resolution]] 3.42Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WBU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WBU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7wbu]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WBU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WBU FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wbu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wbu OCA], [https://pdbe.org/7wbu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wbu RCSB], [https://www.ebi.ac.uk/pdbsum/7wbu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wbu ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wbu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wbu OCA], [https://pdbe.org/7wbu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wbu RCSB], [https://www.ebi.ac.uk/pdbsum/7wbu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wbu ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/NLRP9_BOVIN NLRP9_BOVIN]] As the sensor component of the NLRP9 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens, including rotavirus, initiates the formation of the inflammasome polymeric complex, made of NLRP9, PYCARD and CASP1. Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and release in the extracellular milieu. The active cytokines stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death. NLRP9 inflammasome activation may be initiated by DHX9 interaction with viral double-stranded RNA (dsRNA), preferentially to short dsRNA segments.[UniProtKB:Q7RTR0] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Nucleotide-binding and oligomerisation domain-like receptors (NLRs) can form inflammasomes that activate caspase-1 and pro-interleukin-1beta and induce pyroptosis. NLR family pyrin domain-containing 9 (NLRP9) forms an inflammasome and activates innate immune responses during virus infection, but little is known about this process. Here, we report the crystal and cryo-electron microscopy structures of NLRP9 in an ADP-bound state, revealing inactive and closed conformations of NLRP9 and its similarities to other structurally characterised NLRs. Moreover, we found a C-terminal region interacting with the concave surface of the leucine-rich repeat domain of NLRP9. This region is unique among NLRs and might be involved in the specific function of NLRP9. These data provide the structural basis for understanding the mechanism of NLRP9 regulation and activation. | ||
| + | |||
| + | The structure of NLRP9 reveals a unique C-terminal region with putative regulatory function.,Kamitsukasa Y, Nakano K, Murakami K, Hirata K, Yamamoto M, Shimizu T, Ohto U FEBS Lett. 2022 Jan 28. doi: 10.1002/1873-3468.14302. PMID:35090055<ref>PMID:35090055</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7wbu" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Kamitsukasa Y]] | + | [[Category: Kamitsukasa, Y]] |
| - | [[Category: Ohto U]] | + | [[Category: Ohto, U]] |
| - | [[Category: Shimizu T]] | + | [[Category: Shimizu, T]] |
| + | [[Category: Immune system]] | ||
| + | [[Category: Inflammasome]] | ||
| + | [[Category: Nlr]] | ||
| + | [[Category: Nlrp9]] | ||
| + | [[Category: Nod-like receptor]] | ||
Current revision
Cryo-EM structure of bovine NLRP9
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