2xvm

From Proteopedia

(Difference between revisions)

Revision as of 12:17, 27 April 2022

Crystal structure of the tellurite detoxification protein TehB from E. coli in complex with SAH

Structural highlights

2xvm is a 2 chain structure with sequence from "bacillus_coli"_migula_1895 "bacillus coli" migula 1895. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	2xva
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[TEHB_ECOLI] S-adenosyl-L-methionine dependent methyltransferase that catalyzes the methylation of tellurite and is responsible for tellurite resistance when present in high copy number. Can also methylate selenite and selenium dioxide. Is thus able to detoxify different chalcogens. Can not methylate arsenic compounds.^[1] ^[2]

Publication Abstract from PubMed

The oxyanion derivatives of the chalcogens tellurium and selenium are toxic to living organisms even at very low levels. Bacteria have developed mechanisms to overcome their toxicity by methylating them. The structure of the TehB from Escherichia coli has been determined in the presence of the co-factor analogues S-adenosyl-homocysteine (SAH) and sinefungin (non-hydrolysable form of S-adenosyl-L-methionine) at 1.48 A and 1.9 A respectively. Interestingly, our kinetic data show that TehB does not discriminate between selenium or tellurite oxyanions, making it a very powerful detoxifying protein. Analysis of the active site has identified three conserved residues that are capable of binding and orientating the metals for nucleophilic attack, His176, Arg177 and Arg184. Mutagenesis studies revealed that the His176Ala and Arg184Ala mutants retained most of their activity, whereas the Arg177Ala mutant had 65 % of its activity abolished. Based on the structure and kinetic data we propose an SN2 nucleophilic attack reaction mechanism. These data provide the first molecular understanding on the detoxification of chalcogens by bacteria.

Structure and mechanism of the chalcogen detoxifying protein TehB from Escherichia coli.,Choudhury HG, Cameron AD, Iwata S, Beis K Biochem J. 2011 Jan 18. PMID:21244361^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Liu M, Turner RJ, Winstone TL, Saetre A, Dyllick-Brenzinger M, Jickling G, Tari LW, Weiner JH, Taylor DE. Escherichia coli TehB requires S-adenosylmethionine as a cofactor to mediate tellurite resistance. J Bacteriol. 2000 Nov;182(22):6509-13. PMID:11053398
↑ Choudhury HG, Cameron AD, Iwata S, Beis K. Structure and mechanism of the chalcogen detoxifying protein TehB from Escherichia coli. Biochem J. 2011 Jan 18. PMID:21244361 doi:10.1042/BJ20102014
↑ Choudhury HG, Cameron AD, Iwata S, Beis K. Structure and mechanism of the chalcogen detoxifying protein TehB from Escherichia coli. Biochem J. 2011 Jan 18. PMID:21244361 doi:10.1042/BJ20102014

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2xvm"

@@ Line 3: / Line 3: @@
 <StructureSection load='2xvm' size='340' side='right'caption='[[2xvm]], [[Resolution|resolution]] 1.48&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2xvm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XVM OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2XVM FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2xvm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XVM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XVM FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2xva|2xva]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2xva|2xva]]</div></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2xvm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xvm OCA], [http://pdbe.org/2xvm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xvm RCSB], [http://www.ebi.ac.uk/pdbsum/2xvm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xvm ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xvm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xvm OCA], [https://pdbe.org/2xvm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xvm RCSB], [https://www.ebi.ac.uk/pdbsum/2xvm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xvm ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/TEHB_ECOLI TEHB_ECOLI]] S-adenosyl-L-methionine dependent methyltransferase that catalyzes the methylation of tellurite and is responsible for tellurite resistance when present in high copy number. Can also methylate selenite and selenium dioxide. Is thus able to detoxify different chalcogens. Can not methylate arsenic compounds.<ref>PMID:11053398</ref> <ref>PMID:21244361</ref>
+[[https://www.uniprot.org/uniprot/TEHB_ECOLI TEHB_ECOLI]] S-adenosyl-L-methionine dependent methyltransferase that catalyzes the methylation of tellurite and is responsible for tellurite resistance when present in high copy number. Can also methylate selenite and selenium dioxide. Is thus able to detoxify different chalcogens. Can not methylate arsenic compounds.<ref>PMID:11053398</ref> <ref>PMID:21244361</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==

2xvm

From Proteopedia

Revision as of 12:17, 27 April 2022

Crystal structure of the tellurite detoxification protein TehB from E. coli in complex with SAH

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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