7t3p
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==IP3 and ATP bound type 3 IP3 receptor in the pre-active A state== | ==IP3 and ATP bound type 3 IP3 receptor in the pre-active A state== | ||
| - | <StructureSection load='7t3p' size='340' side='right'caption='[[7t3p]]' scene=''> | + | <StructureSection load='7t3p' size='340' side='right'caption='[[7t3p]], [[Resolution|resolution]] 3.20Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T3P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T3P FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7t3p]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T3P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T3P FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t3p OCA], [https://pdbe.org/7t3p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t3p RCSB], [https://www.ebi.ac.uk/pdbsum/7t3p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t3p ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=I3P:D-MYO-INOSITOL-1,4,5-TRIPHOSPHATE'>I3P</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t3p OCA], [https://pdbe.org/7t3p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t3p RCSB], [https://www.ebi.ac.uk/pdbsum/7t3p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t3p ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/ITPR3_HUMAN ITPR3_HUMAN]] Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A pivotal component of the calcium (Ca(2+)) signaling toolbox in cells is the inositol 1,4,5-triphosphate (IP3) receptor (IP3R), which mediates Ca(2+) release from the endoplasmic reticulum (ER), controlling cytoplasmic and organellar Ca(2+) concentrations. IP3Rs are co-activated by IP3 and Ca(2+), inhibited by Ca(2+) at high concentrations, and potentiated by ATP. However, the underlying molecular mechanisms are unclear. Here we report cryo-electron microscopy (cryo-EM) structures of human type-3 IP3R obtained from a single dataset in multiple gating conformations: IP3-ATP bound pre-active states with closed channels, IP3-ATP-Ca(2+) bound active state with an open channel, and IP3-ATP-Ca(2+) bound inactive state with a closed channel. The structures demonstrate how IP3-induced conformational changes prime the receptor for activation by Ca(2+), how Ca(2+) binding leads to channel opening, and how ATP modulates the activity, providing insights into the long-sought questions regarding the molecular mechanism underpinning receptor activation and gating. | ||
| + | |||
| + | Structural basis for activation and gating of IP3 receptors.,Schmitz EA, Takahashi H, Karakas E Nat Commun. 2022 Mar 17;13(1):1408. doi: 10.1038/s41467-022-29073-2. PMID:35301323<ref>PMID:35301323</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7t3p" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Karakas E]] | + | [[Category: Karakas, E]] |
| - | [[Category: Schmitz | + | [[Category: Schmitz, E A]] |
| - | [[Category: Takahashi H]] | + | [[Category: Takahashi, H]] |
| + | [[Category: Calcium signaling]] | ||
| + | [[Category: Ip3 receptor]] | ||
| + | [[Category: Metal transport]] | ||
Revision as of 13:08, 4 May 2022
IP3 and ATP bound type 3 IP3 receptor in the pre-active A state
| |||||||||||
