Journal:Acta Cryst D:S2059798319009008
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In a random microseed matrix, a crystal or crystalline precipitate is crushed up, diluted and added to new crystallization conditions from a sparse matrix screen. This often generates new crystallization where crystals can grow. I3C is compatible with many crystallization reagents and provides a large phasing power. | In a random microseed matrix, a crystal or crystalline precipitate is crushed up, diluted and added to new crystallization conditions from a sparse matrix screen. This often generates new crystallization where crystals can grow. I3C is compatible with many crystallization reagents and provides a large phasing power. | ||
| - | In the two test proteins, the <scene name='81/819238/Cv/2'>Orf11 NTD</scene> from ''Staphylococcus'' phage P68 and <scene name='81/819238/Cv/3'>Hen Egg White Lysozyme</scene>, the rMMS technique in the presence of I3C generated numerous new conditions where derivatized crystals could grow. The I3C molecules in the lattice were sufficient to completely solve the structure using anomalous phasing methods. | + | In the two test proteins, the <scene name='81/819238/Cv/2'>Orf11 NTD</scene> from ''Staphylococcus'' phage P68 (PDB entry [[6o43]]) and <scene name='81/819238/Cv/3'>Hen Egg White Lysozyme</scene> (PDB entry [[6pbb]]), the rMMS technique in the presence of I3C generated numerous new conditions where derivatized crystals could grow. The I3C molecules in the lattice were sufficient to completely solve the structure using anomalous phasing methods. |
I3C was present in the tested crystals in the rMMS screens containing I3C, where they often sit at the junctions between protein molecules in the lattice. These bridging contacts could improve the crystal lattice or generate new conditions where crystals can grow. | I3C was present in the tested crystals in the rMMS screens containing I3C, where they often sit at the junctions between protein molecules in the lattice. These bridging contacts could improve the crystal lattice or generate new conditions where crystals can grow. | ||
Revision as of 12:27, 10 May 2022
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This page complements a publication in scientific journals and is one of the Proteopedia's Interactive 3D Complement pages. For aditional details please see I3DC.
