7ep9
From Proteopedia
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==The structure of carboxypeptidase from Fusobacterium nucleatum== | ==The structure of carboxypeptidase from Fusobacterium nucleatum== | ||
| - | <StructureSection load='7ep9' size='340' side='right'caption='[[7ep9]]' scene=''> | + | <StructureSection load='7ep9' size='340' side='right'caption='[[7ep9]], [[Resolution|resolution]] 2.60Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EP9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EP9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7ep9]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EP9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EP9 FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ep9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ep9 OCA], [https://pdbe.org/7ep9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ep9 RCSB], [https://www.ebi.ac.uk/pdbsum/7ep9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ep9 ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ep9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ep9 OCA], [https://pdbe.org/7ep9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ep9 RCSB], [https://www.ebi.ac.uk/pdbsum/7ep9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ep9 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | As one of the most abundant bacteria in the human oral cavity, Fusobacterium nucleatum is closely involved in various oral diseases and is also a risk factor for other diseases. The peptidases of F. nucleatum can digest exogenous peptides into amino acids to satisfy its nutrient requirements. Here, a putative F. nucleatum peptidase, termed S9Cfn, which belongs to the S9C peptidase family was identified. Enzymatic activity assays combined with mass-spectrometric analysis revealed that S9Cfn is a carboxypeptidase, but not an aminopeptidase as previously annotated. The crystal structure of the S9Cfn tetramer was solved at 2.6 A resolution and was found to contain a pair of oligomeric pores in the center. Structural analysis, together with site-directed mutagenesis and enzymatic activity assays, revealed a substrate-entrance tunnel that extends from each oligomeric pore to the catalytic triad, adjacent to which three conserved arginine residues are responsible for substrate binding. Moreover, comparison with other S9 peptidase structures indicated drastic conformational changes of the oligomeric pores during the catalytic cycle. Together, these findings increase the knowledge of this unique type of tetrameric carboxypeptidase and provide insight into the homeostatic control of microbiota in the human oral cavity. | ||
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| + | Structural and biochemical analyses of the tetrameric carboxypeptidase S9Cfn from Fusobacterium nucleatum.,Wang X, Cheng MT, Chen ZP, Jiang YL, Ge YS, Xia R, Hou WT Acta Crystallogr D Struct Biol. 2021 Dec 1;77(Pt 12):1554-1563. doi:, 10.1107/S2059798321010810. Epub 2021 Nov 17. PMID:34866611<ref>PMID:34866611</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7ep9" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Jiang | + | [[Category: Jiang, Y L]] |
| - | [[Category: Wang X]] | + | [[Category: Wang, X]] |
| + | [[Category: Crystal screening]] | ||
| + | [[Category: Fusobacterium nucleatum]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Serine peptidase]] | ||
Revision as of 06:26, 12 May 2022
The structure of carboxypeptidase from Fusobacterium nucleatum
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