|
|
| Line 1: |
Line 1: |
| | | | |
| | ==Crystal structure of human Maf1== | | ==Crystal structure of human Maf1== |
| - | <StructureSection load='3nr5' size='340' side='right' caption='[[3nr5]], [[Resolution|resolution]] 1.55Å' scene=''> | + | <StructureSection load='3nr5' size='340' side='right'caption='[[3nr5]], [[Resolution|resolution]] 1.55Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3nr5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NR5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NR5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3nr5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NR5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NR5 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MAF1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MAF1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nr5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nr5 OCA], [http://pdbe.org/3nr5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3nr5 RCSB], [http://www.ebi.ac.uk/pdbsum/3nr5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3nr5 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nr5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nr5 OCA], [https://pdbe.org/3nr5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nr5 RCSB], [https://www.ebi.ac.uk/pdbsum/3nr5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nr5 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MAF1_HUMAN MAF1_HUMAN]] Element of the mTORC1 signaling pathway that acts as a mediator of diverse signals and that represses RNA polymerase III transcription. Inhibits the de novo assembly of TFIIIB onto DNA.<ref>PMID:20233713</ref> <ref>PMID:20516213</ref> <ref>PMID:20543138</ref> | + | [[https://www.uniprot.org/uniprot/MAF1_HUMAN MAF1_HUMAN]] Element of the mTORC1 signaling pathway that acts as a mediator of diverse signals and that represses RNA polymerase III transcription. Inhibits the de novo assembly of TFIIIB onto DNA.<ref>PMID:20233713</ref> <ref>PMID:20516213</ref> <ref>PMID:20543138</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 23: |
Line 23: |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Human]] | | [[Category: Human]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Berninghausen, O]] | | [[Category: Berninghausen, O]] |
| | [[Category: Cramer, P]] | | [[Category: Cramer, P]] |
| Structural highlights
Function
[MAF1_HUMAN] Element of the mTORC1 signaling pathway that acts as a mediator of diverse signals and that represses RNA polymerase III transcription. Inhibits the de novo assembly of TFIIIB onto DNA.[1] [2] [3]
Publication Abstract from PubMed
RNA polymerase III (Pol III) transcribes short RNAs required for cell growth. Under stress conditions, the conserved protein Maf1 rapidly represses Pol III transcription. We report the crystal structure of Maf1 and cryo-electron microscopic structures of Pol III, an active Pol III-DNA-RNA complex, and a repressive Pol III-Maf1 complex. Binding of DNA and RNA causes ordering of the Pol III-specific subcomplex C82/34/31 that is required for transcription initiation. Maf1 binds the Pol III clamp and rearranges C82/34/31 at the rim of the active center cleft. This impairs recruitment of Pol III to a complex of promoter DNA with the initiation factors Brf1 and TBP and thus prevents closed complex formation. Maf1 does however not impair binding of a DNA-RNA scaffold and RNA synthesis. These results explain how Maf1 specifically represses transcription initiation from Pol III promoters and indicate that Maf1 also prevents reinitiation by binding Pol III during transcription elongation.
Molecular basis of RNA polymerase III transcription repression by Maf1.,Vannini A, Ringel R, Kusser AG, Berninghausen O, Kassavetis GA, Cramer P Cell. 2010 Oct 1;143(1):59-70. PMID:20887893[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Shor B, Wu J, Shakey Q, Toral-Barza L, Shi C, Follettie M, Yu K. Requirement of the mTOR kinase for the regulation of Maf1 phosphorylation and control of RNA polymerase III-dependent transcription in cancer cells. J Biol Chem. 2010 May 14;285(20):15380-92. doi: 10.1074/jbc.M109.071639. Epub, 2010 Mar 16. PMID:20233713 doi:10.1074/jbc.M109.071639
- ↑ Michels AA, Robitaille AM, Buczynski-Ruchonnet D, Hodroj W, Reina JH, Hall MN, Hernandez N. mTORC1 directly phosphorylates and regulates human MAF1. Mol Cell Biol. 2010 Aug;30(15):3749-57. doi: 10.1128/MCB.00319-10. Epub 2010 Jun , 1. PMID:20516213 doi:10.1128/MCB.00319-10
- ↑ Kantidakis T, Ramsbottom BA, Birch JL, Dowding SN, White RJ. mTOR associates with TFIIIC, is found at tRNA and 5S rRNA genes, and targets their repressor Maf1. Proc Natl Acad Sci U S A. 2010 Jun 29;107(26):11823-8. doi:, 10.1073/pnas.1005188107. Epub 2010 Jun 11. PMID:20543138 doi:10.1073/pnas.1005188107
- ↑ Vannini A, Ringel R, Kusser AG, Berninghausen O, Kassavetis GA, Cramer P. Molecular basis of RNA polymerase III transcription repression by Maf1. Cell. 2010 Oct 1;143(1):59-70. PMID:20887893 doi:10.1016/j.cell.2010.09.002
|
