3o15

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==Crystal Structure of Bacillus subtilis Thiamin Phosphate Synthase Complexed with a Carboxylated Thiazole Phosphate==
==Crystal Structure of Bacillus subtilis Thiamin Phosphate Synthase Complexed with a Carboxylated Thiazole Phosphate==
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<StructureSection load='3o15' size='340' side='right' caption='[[3o15]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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<StructureSection load='3o15' size='340' side='right'caption='[[3o15]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3o15]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O15 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O15 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3o15]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O15 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O15 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3NM:4-METHYL-5-[2-(PHOSPHONOOXY)ETHYL]-1,3-THIAZOLE-2-CARBOXYLIC+ACID'>3NM</scene>, <scene name='pdbligand=IFP:2-TRIFLUOROMETHYL-5-METHYLENE-5H-PYRIMIDIN-4-YLIDENEAMINE'>IFP</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene></td></tr>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3NM:4-METHYL-5-[2-(PHOSPHONOOXY)ETHYL]-1,3-THIAZOLE-2-CARBOXYLIC+ACID'>3NM</scene>, <scene name='pdbligand=IFP:2-TRIFLUOROMETHYL-5-METHYLENE-5H-PYRIMIDIN-4-YLIDENEAMINE'>IFP</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3o16|3o16]]</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3o16|3o16]]</div></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">thiE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">thiE ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thiamine-phosphate_diphosphorylase Thiamine-phosphate diphosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.3 2.5.1.3] </span></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Thiamine-phosphate_diphosphorylase Thiamine-phosphate diphosphorylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.3 2.5.1.3] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o15 OCA], [http://pdbe.org/3o15 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3o15 RCSB], [http://www.ebi.ac.uk/pdbsum/3o15 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3o15 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o15 OCA], [https://pdbe.org/3o15 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o15 RCSB], [https://www.ebi.ac.uk/pdbsum/3o15 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o15 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/THIE_BACSU THIE_BACSU]] Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP). Is also able to use the 2-methoxy analog MeO-HMP-PP, as substrate in vitro, but not the 2-trifluoromethyl analog CF(3)-HMP-PP.<ref>PMID:9139923</ref> <ref>PMID:11513588</ref>
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[[https://www.uniprot.org/uniprot/THIE_BACSU THIE_BACSU]] Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP). Is also able to use the 2-methoxy analog MeO-HMP-PP, as substrate in vitro, but not the 2-trifluoromethyl analog CF(3)-HMP-PP.<ref>PMID:9139923</ref> <ref>PMID:11513588</ref>
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==See Also==
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*[[Thiamin phosphate synthase|Thiamin phosphate synthase]]
== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Vibrio subtilis ehrenberg 1835]]
[[Category: Vibrio subtilis ehrenberg 1835]]
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[[Category: Large Structures]]
[[Category: Thiamine-phosphate diphosphorylase]]
[[Category: Thiamine-phosphate diphosphorylase]]
[[Category: Abdelwahed, S]]
[[Category: Abdelwahed, S]]

Revision as of 07:13, 12 May 2022

Crystal Structure of Bacillus subtilis Thiamin Phosphate Synthase Complexed with a Carboxylated Thiazole Phosphate

PDB ID 3o15

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