Transketolase

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<StructureSection load='' size='350' side='right' caption='E. coli transketolase 1 dimer complex with xylulose-5-phosphate-thiamine diphosphate adduct, ethylene glycol and Ca+2 ion (green) (PDB code [[2r8o]]).' scene='46/466534/Cv/1'>
<StructureSection load='' size='350' side='right' caption='E. coli transketolase 1 dimer complex with xylulose-5-phosphate-thiamine diphosphate adduct, ethylene glycol and Ca+2 ion (green) (PDB code [[2r8o]]).' scene='46/466534/Cv/1'>
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_TOC_
 
== Function ==
== Function ==
'''Transketolase''' (TKT) catalyzes two opposite reactions. The synthesis of sedoheptulose-7-P in the pentose phosphate pathway using thiamine diphosphate (TPP) as co-factor; and the conversion of sedoheptulose-7-P and glyceraldehyde-3-P to aldose and ketose in the Calvin cycle<ref>PMID:8369276</ref>. '''Split-gene TKT''' is found in the hyperthermophilic bacterium ''Carboxydothermus hydrogenoformans'' and is reconstituted from 2 separate polypeptide chains<ref>PMID:33193259</ref>.
'''Transketolase''' (TKT) catalyzes two opposite reactions. The synthesis of sedoheptulose-7-P in the pentose phosphate pathway using thiamine diphosphate (TPP) as co-factor; and the conversion of sedoheptulose-7-P and glyceraldehyde-3-P to aldose and ketose in the Calvin cycle<ref>PMID:8369276</ref>. '''Split-gene TKT''' is found in the hyperthermophilic bacterium ''Carboxydothermus hydrogenoformans'' and is reconstituted from 2 separate polypeptide chains<ref>PMID:33193259</ref>.
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The <scene name='46/466534/Cv/7'>active site of TKT contains a ligand-cofactor adduct</scene>.<ref>PMID:17914867</ref> Water molecules are shown as red spheres. <scene name='46/466534/Cv/8'>Ca coordination site</scene>.
The <scene name='46/466534/Cv/7'>active site of TKT contains a ligand-cofactor adduct</scene>.<ref>PMID:17914867</ref> Water molecules are shown as red spheres. <scene name='46/466534/Cv/8'>Ca coordination site</scene>.
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[[Transketolase 3D structures]]
 
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</StructureSection>
 
== 3D Structures of transketolase ==
== 3D Structures of transketolase ==
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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[[Transketolase 3D structures]]
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{{#tree:id=OrganizedByTopic|openlevels=0|
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</StructureSection>
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* Transketolase
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**[[1trk]] – yTKT – yeast
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**[[1ay0]] – yTKT (mutant)
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**[[2e6k]] – TKT – ''Thermus thermophilus''
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**[[3hyl]] – BaTKT – ''Bacillus anthracis''
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**[[3kom]] – TKT (mutant) – ''Francisella tularensis''
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**[[3l84]] – CjTKT – ''Campylobacter jejuni''<br />
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**[[3rim]] – TKT – ''Mycobacterium tuberculosis''<br />
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**[[3uk1]] – BtTKT – ''Burkholderia thailandensis''<br />
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**[[4c7v]] - LsTKT – ''Lactobacillus salivarius''<br />
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**[[4xeu]] - TKT – ''Pseudomonas aeruginosa''<br />
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**[[5i4i]], [[5xry]] - PsTKT – ''Pichia stipitis''<br />
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**[[5hgx]], [[5i5g]] - PsTKT (mutant)<br />
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**[[5nd6]] - CrTKT – ''Chlamydomonas reinhardtii''<br />
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**[[5vrb]] - TKT – ''Neisseria gonorrhoeae''<br />
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**[[6rjc]] - EcTKT 1 – ''Escherichia coli''<br />
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*Transketolase binary complexes
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**[[3mos]], [[3ooy]] – hTKT + TPP - human<br />
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**[[4kxy]] - hTKT + TPP analog<br />
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**[[6ha3]] - hTKT (mutant) + fructose phosphate/TPP<br />
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**[[6had]], [[6rjb]] - hTKT (mutant) + TDP<br />
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**[[1itz]] - TKT + TPP – maize
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**[[1tka]], [[1tkb]], [[1tkc]] – yTKT + TPP analog
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**[[1gpu]] - yTKT 1 + TPP derivative
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**[[1qgd]] - EcTKT + TPP <br />
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**[[6tj8]] - EcTKT 1 + TPP analog <br />
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**[[5hht]] - EcTKT (mutant) + TDP <br />
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**[[1r9j]] - TKT + TPP – ''Leishmania mexicana''<br />
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**[[4c7x]] - LsTKT + TPP <br />
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**[[5hyv]], [[5x56]], [[5xuf]], [[5xvt]], [[5xs6]] - PsTKT + TPP <br />
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**[[5xsa]], [[5xsb]], [[5xsm]], [[5xt0]], [[5xt4]], [[5xtv]], [[5xtx]] - PsTKT + TPP intermediate<br />
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**[[5xqk]] - PsTKT + xylulose-5-P<br />
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**[[5xqa]] - PsTKT + ribose-5-P<br />
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**[[5xps]] - PsTKT + erythrose-4-P<br />
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**[[5xrv]] - PsTKT + fructose-6-P<br />
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**[[5i51]] - PsTKT (mutant) + fructose-6-P<br />
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**[[5xtl]] - PsTKT + pyrimidine derivative <br />
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**[[5nd5]] - CrTKT + TPP <br />
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**[[3m49]] – BaTKT + TPP<br />
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*Transketolase ternary complexes
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**[[4kxu]] - hTKT + TPP + sorbitol 6-P<br />
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**[[4kxv]], [[4kxw]] - hTKT + TPP + xylitol 5-P<br />
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**[[4kxx]] - hTKT + TPP + seduheptulose 7-P<br />
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**[[1ngs]] - yTKT + TPP + Ca + erythrose-4-P<br />
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**[[3m7i]], [[3m6l]] – CjTKT + TPP + ribose 5-P
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**[[3m34]] - CjTKT + TPP + Ca
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**[[2r5n]] – EcTKT 1 + TPP + ribose 5-P<br />
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**[[3upt]],[[5xqa]] - BtTKT + TPP + ribose 5-P<br />
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**[[2r8o]], [[5xqk]] - EcTKT 1 + TPP + xylulose 5-P<br />
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**[[6tj9]] - EcTKT 1 + TPP analog + xylulose 5-P<br />
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**[[2r8p]] - EcTKT 1 + TPP + fructose 6-P<br />
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**[[5hje]] - PsTKT + TPP + seduheptulose 7-P<br />
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**[[5i51]], [[5xrv]], [[5xu2]] - PsTKT + TPP + fructose 6-P<br />
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**[[5xps]], [[5xu9]] - PsTKT + TPP + erythrose 4-P<br />
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**[[5i5e]] - PsTKT (mutant) + TPP + xylulose 5-P<br />
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*Split-gene transketolase
 
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**[[6yak]] - ChTKT N-terminal + C-terminal chain – ''Carboxydothermus hydrogenoformans''<br />
 
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**[[6yaj]] - ChTKT C-terminal chain<br />
 
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}}
 
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Revision as of 07:30, 16 May 2022

E. coli transketolase 1 dimer complex with xylulose-5-phosphate-thiamine diphosphate adduct, ethylene glycol and Ca+2 ion (green) (PDB code 2r8o).

Drag the structure with the mouse to rotate


References

  1. ↑ Verschueren KH, Kingma J, Rozeboom HJ, Kalk KH, Janssen DB, Dijkstra BW. Crystallographic and fluorescence studies of the interaction of haloalkane dehalogenase with halide ions. Studies with halide compounds reveal a halide binding site in the active site. Biochemistry. 1993 Sep 7;32(35):9031-7. PMID:8369276
  2. ↑ James P, Isupov MN, De Rose SA, Sayer C, Cole IS, Littlechild JA. A 'Split-Gene' Transketolase From the Hyper-Thermophilic Bacterium Carboxydothermus hydrogenoformans: Structure and Biochemical Characterization. Front Microbiol. 2020 Oct 30;11:592353. doi: 10.3389/fmicb.2020.592353., eCollection 2020. PMID:33193259 doi:http://dx.doi.org/10.3389/fmicb.2020.592353
  3. ↑ Asztalos P, Parthier C, Golbik R, Kleinschmidt M, Hubner G, Weiss MS, Friedemann R, Wille G, Tittmann K. Strain and near attack conformers in enzymic thiamin catalysis: X-ray crystallographic snapshots of bacterial transketolase in covalent complex with donor ketoses xylulose 5-phosphate and fructose 6-phosphate, and in noncovalent complex with acceptor aldose ribose 5-phosphate. Biochemistry. 2007 Oct 30;46(43):12037-52. Epub 2007 Oct 3. PMID:17914867 doi:10.1021/bi700844m

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