1h4i

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[[Image:1h4i.gif|left|200px]]
[[Image:1h4i.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1h4i |SIZE=350|CAPTION= <scene name='initialview01'>1h4i</scene>, resolution 1.94&Aring;
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The line below this paragraph, containing "STRUCTURE_1h4i", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Pqq+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Ca+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Pqq+Binding+Site+For+Chain+C'>AC3</scene> and <scene name='pdbsite=AC4:Ca+Binding+Site+For+Chain+C'>AC4</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PQQ:PYRROLOQUINOLINE+QUINONE'>PQQ</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Alcohol_dehydrogenase_(acceptor) Alcohol dehydrogenase (acceptor)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.99.8 1.1.99.8] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1h4i| PDB=1h4i | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h4i OCA], [http://www.ebi.ac.uk/pdbsum/1h4i PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1h4i RCSB]</span>
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}}
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'''METHYLOBACTERIUM EXTORQUENS METHANOL DEHYDROGENASE'''
'''METHYLOBACTERIUM EXTORQUENS METHANOL DEHYDROGENASE'''
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==Reference==
==Reference==
The refined structure of the quinoprotein methanol dehydrogenase from Methylobacterium extorquens at 1.94 A., Ghosh M, Anthony C, Harlos K, Goodwin MG, Blake C, Structure. 1995 Feb 15;3(2):177-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7735834 7735834]
The refined structure of the quinoprotein methanol dehydrogenase from Methylobacterium extorquens at 1.94 A., Ghosh M, Anthony C, Harlos K, Goodwin MG, Blake C, Structure. 1995 Feb 15;3(2):177-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7735834 7735834]
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[[Category: Alcohol dehydrogenase (acceptor)]]
 
[[Category: Methylobacterium extorquens]]
[[Category: Methylobacterium extorquens]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: Goodwin, M G.]]
[[Category: Goodwin, M G.]]
[[Category: Harlos, K.]]
[[Category: Harlos, K.]]
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[[Category: dehydrogenase]]
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[[Category: Dehydrogenase]]
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[[Category: quinoprotein]]
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[[Category: Quinoprotein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:25:14 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:57:33 2008''
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Revision as of 15:25, 2 May 2008

Image:1h4i.gif

Template:STRUCTURE 1h4i

METHYLOBACTERIUM EXTORQUENS METHANOL DEHYDROGENASE


Overview

BACKGROUND: Methanol dehydrogenase (MDH) is a bacterial periplasmic quinoprotein; it has pyrrolo-quinoline quinone (PQQ) as its prosthetic group, requires Ca2+ for activity and uses cytochrome cL as its electron acceptor. Low-resolution structures of MDH have already been determined. RESULTS: The structure of the alpha 2 beta 2 tetramer of MDH from Methylobacterium extorquens has now been determined at 1.94 A with an R-factor of 19.85%. CONCLUSIONS: The alpha-subunit of MDH has an eight-fold radial symmetry, with its eight beta-sheets stabilized by a novel tryptophan docking motif. The PQQ in the active site is held in place by a coplanar tryptophan and by a novel disulphide ring formed between adjacent cysteines which are bonded by an unusual non-planar trans peptide bond. One of the carbonyl oxygens of PQQ is bonded to the Ca2+, probably facilitating attack on the substrate, and the other carbonyl oxygen is out of the plane of the ring, confirming the presence of the predicted free-radical semiquinone form of the prosthetic group.

About this Structure

1H4I is a Protein complex structure of sequences from Methylobacterium extorquens. Full crystallographic information is available from OCA.

Reference

The refined structure of the quinoprotein methanol dehydrogenase from Methylobacterium extorquens at 1.94 A., Ghosh M, Anthony C, Harlos K, Goodwin MG, Blake C, Structure. 1995 Feb 15;3(2):177-87. PMID:7735834 Page seeded by OCA on Fri May 2 18:25:14 2008

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