1h4m

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[[Image:1h4m.gif|left|200px]]
[[Image:1h4m.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1h4m |SIZE=350|CAPTION= <scene name='initialview01'>1h4m</scene>, resolution 2.1&Aring;
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The line below this paragraph, containing "STRUCTURE_1h4m", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Thiosulfate_sulfurtransferase Thiosulfate sulfurtransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.1.1 2.8.1.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= RHDA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=354 Azotobacter vinelandii])
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-->
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|DOMAIN=
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{{STRUCTURE_1h4m| PDB=1h4m | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h4m OCA], [http://www.ebi.ac.uk/pdbsum/1h4m PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1h4m RCSB]</span>
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}}
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'''SULFURTRANSFERASE FROM AZOTOBACTER VINELANDII IN COMPLEX WITH PHOSPHATE'''
'''SULFURTRANSFERASE FROM AZOTOBACTER VINELANDII IN COMPLEX WITH PHOSPHATE'''
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[[Category: Pagani, S.]]
[[Category: Pagani, S.]]
[[Category: Spallarossa, A.]]
[[Category: Spallarossa, A.]]
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[[Category: sulfur metabolism]]
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[[Category: Sulfur metabolism]]
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[[Category: sulfurtransferase]]
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[[Category: Sulfurtransferase]]
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[[Category: thiosulfate:cyanide]]
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[[Category: Thiosulfate:cyanide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:25:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:57:33 2008''
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Revision as of 15:25, 2 May 2008

Image:1h4m.gif

Template:STRUCTURE 1h4m

SULFURTRANSFERASE FROM AZOTOBACTER VINELANDII IN COMPLEX WITH PHOSPHATE


Overview

Active site reactivity and specificity of RhdA, a thiosulfate:cyanide sulfurtransferase (rhodanese) from Azotobacter vinelandii, have been investigated through ligand binding, site-directed mutagenesis, and X-ray crystallographic techniques, in a combined approach. In native RhdA the active site Cys230 is found persulfurated; fluorescence and sulfurtransferase activity measurements show that phosphate anions interact with Cys230 persulfide sulfur atom and modulate activity. Crystallographic analyses confirm that phosphate and hypophosphite anions react with native RhdA, removing the persulfide sulfur atom from the active site pocket. Considering that RhdA and the catalytic subunit of Cdc25 phosphatases share a common three-dimensional fold as well as active site Cys (catalytic) and Arg residues, two RhdA mutants carrying a single amino acid insertion at the active site loop were designed and their phosphatase activity tested. The crystallographic and functional results reported here show that specific sulfurtransferase or phosphatase activities are strictly related to precise tailoring of the catalytic loop structure in RhdA and Cdc25 phosphatase, respectively.

About this Structure

1H4M is a Single protein structure of sequence from Azotobacter vinelandii. Full crystallographic information is available from OCA.

Reference

A persulfurated cysteine promotes active site reactivity in Azotobacter vinelandii Rhodanese., Bordo D, Forlani F, Spallarossa A, Colnaghi R, Carpen A, Bolognesi M, Pagani S, Biol Chem. 2001 Aug;382(8):1245-52. PMID:11592406 Page seeded by OCA on Fri May 2 18:25:34 2008

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