7awk
From Proteopedia
(Difference between revisions)
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==Crystal structure of the HigB1 toxin mutant K95A from Mycobacterium tuberculosis (Rv1955)== | ==Crystal structure of the HigB1 toxin mutant K95A from Mycobacterium tuberculosis (Rv1955)== | ||
| - | <StructureSection load='7awk' size='340' side='right'caption='[[7awk]]' scene=''> | + | <StructureSection load='7awk' size='340' side='right'caption='[[7awk]], [[Resolution|resolution]] 1.91Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AWK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AWK FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7awk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AWK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AWK FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7awk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7awk OCA], [https://pdbe.org/7awk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7awk RCSB], [https://www.ebi.ac.uk/pdbsum/7awk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7awk ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7awk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7awk OCA], [https://pdbe.org/7awk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7awk RCSB], [https://www.ebi.ac.uk/pdbsum/7awk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7awk ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/HIGB1_MYCTU HIGB1_MYCTU]] Toxic component of an atypical, type II toxin-antitoxin chaperone (TAC) system. Upon expression in M.smegmatis inhibits colony formation and cell growth (PubMed:20011113, PubMed:23927792). Ectopic expression in wild-type M.tuberculosis has no effect on cell growth; ectopic expression in a triple higB1-higA1-Rv1957 (delta TAC) disruption mutant causes growth arrest, killing a considerable proportion of the cells. Increased ectopic expression leads to decreased levels of IdeR- and Zur-regulated genes as well as cleavage within the mRNA region of tmRNA (transfer-mRNA), strongly suggesting it is an endoribonuclease; also degrades E.coli and M-smegmatis tmRNA (PubMed:23927792). Its toxic effect is neutralized by coexpression with antitoxin HigA. Neutralization of HigB1 toxin in E.coli or M.marinum also requires SecB-like chaperone Rv1957, making this the first toxin-antitoxin chaperone (TAC) system (PubMed:21536872).<ref>PMID:19016878</ref> <ref>PMID:20011113</ref> <ref>PMID:21536872</ref> <ref>PMID:23927792</ref> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Bigot | + | [[Category: Bigot, D J]] |
| - | [[Category: Guillet V]] | + | [[Category: Guillet, V]] |
| - | [[Category: Mourey L]] | + | [[Category: Mourey, L]] |
| + | [[Category: Mycobacterium tuberculosis]] | ||
| + | [[Category: Rnase]] | ||
| + | [[Category: Toxin]] | ||
| + | [[Category: Toxin-antitoxin-chaperone]] | ||
Revision as of 10:20, 18 May 2022
Crystal structure of the HigB1 toxin mutant K95A from Mycobacterium tuberculosis (Rv1955)
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