7pr2
From Proteopedia
(Difference between revisions)
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==Cocrystal of cytochrome c and sulfonato-thiacalix[4]arene== | ==Cocrystal of cytochrome c and sulfonato-thiacalix[4]arene== | ||
| - | <StructureSection load='7pr2' size='340' side='right'caption='[[7pr2]]' scene=''> | + | <StructureSection load='7pr2' size='340' side='right'caption='[[7pr2]], [[Resolution|resolution]] 1.73Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PR2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PR2 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7pr2]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PR2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PR2 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pr2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pr2 OCA], [https://pdbe.org/7pr2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pr2 RCSB], [https://www.ebi.ac.uk/pdbsum/7pr2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pr2 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=80M:sulfonato-thiacalix[4]arene'>80M</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pr2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pr2 OCA], [https://pdbe.org/7pr2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pr2 RCSB], [https://www.ebi.ac.uk/pdbsum/7pr2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pr2 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/CYC1_YEAST CYC1_YEAST]] Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Controlled protein assembly provides a means to generate biomaterials. Synthetic macrocycles such as the water-soluble sulfonato-calix[n]arenes are useful mediators of protein assembly. Sulfonato-thiacalix[4]arene (tsclx 4 ), with its metal-binding capacity, affords the potential for simultaneous macrocycle- and metal-mediated protein assembly. Here, we describe the tsclx 4 -/Zn-directed assembly of two proteins: cationic alpha-helical cytochrome c (cyt c) and neutral beta-propeller Ralstonia solanacearum lectin (RSL). Two co-crystal forms were obtained with cyt c, each involving multinuclear zinc sites supported by the cone conformation of tsclx 4 . The tsclx 4 /Zn cluster acted as an assembly node via both lysine encapsulation and metal-mediated protein-protein contacts. In the case of RSL, tsclx 4 adopted the 1,2-alternate conformation and supported a dinuclear zinc site with concomitant encapsulation and metal-binding of two histidine side chains. These results, together with the knowledge of thiacalixarene/metal nanoclusters, suggest promising applications for thiacalixarenes in biomaterials and MOF fabrication. | ||
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| + | Protein Frameworks with Thiacalixarene and Zinc.,Flood RJ, Ramberg KO, Mengel DB, Guagnini F, Crowley PB Cryst Growth Des. 2022 May 4;22(5):3271-3276. doi: 10.1021/acs.cgd.2c00108. Epub , 2022 Feb 22. PMID:35529063<ref>PMID:35529063</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7pr2" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Crowley | + | [[Category: Crowley, P B]] |
| - | [[Category: Flood | + | [[Category: Flood, R J]] |
| - | [[Category: Guagnini F]] | + | [[Category: Guagnini, F]] |
| - | [[Category: Ramberg K]] | + | [[Category: Ramberg, K]] |
| + | [[Category: Alpha helix]] | ||
| + | [[Category: Calixarene]] | ||
| + | [[Category: Electron transport]] | ||
| + | [[Category: Molecular glue]] | ||
| + | [[Category: Synthetic receptor]] | ||
| + | [[Category: Thiacalixarene]] | ||
Revision as of 10:23, 18 May 2022
Cocrystal of cytochrome c and sulfonato-thiacalix[4]arene
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