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3ofw

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Revision as of 10:42, 18 May 2022

Crystal structure of recombinant Kunitz Type serine protease Inhibitor-1 from the Carribean sea anemone stichodactyla helianthus

Structural highlights

3ofw is a 1 chain structure with sequence from Stihl. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	3m7q
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ISH1_STOHE] Active against serine, cysteine, and aspartic proteinases. Can bind vertebrate trypsin and chymotrypsin.^[1] ^[2]

Publication Abstract from PubMed

The BPTI/Kunitz-type inhibitor family includes several extremely potent serine protease inhibitors. To date, the inhibitory mechanisms have only been studied for mammalian inhibitors. Here, the first crystal structure of a BPTI/Kunitz-type inhibitor from a marine invertebrate (rShPI-1A) is reported to 2.5 A resolution. Crystallization of recombinant rShPI-1A required the salt-induced dissociation of a trypsin complex that was previously formed to avoid intrinsic inhibitor aggregates in solution. The rShPI-1A structure is similar to the NMR structure of the molecule purified from the natural source, but allowed the assignment of disulfide-bridge chiralities and the detection of an internal stabilizing water network. A structural comparison with other BPTI/Kunitz-type canonical inhibitors revealed unusual varphi angles at positions 17 and 30 to be a particular characteristic of the family. A significant clustering of varphi and psi angle values in the glycine-rich remote fragment near the secondary binding loop was additionally identified, but its impact on the specificity of rShPI-1A and similar molecules requires further study.

Structure of the recombinant BPTI/Kunitz-type inhibitor rShPI-1A from the marine invertebrate Stichodactyla helianthus.,Garcia-Fernandez R, Pons T, Meyer A, Perbandt M, Gonzalez-Gonzalez Y, Gil D, de los Angeles Chavez M, Betzel C, Redecke L Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Nov 1;68(Pt 11):1289-93., doi: 10.1107/S1744309112039085. Epub 2012 Oct 26. PMID:23143234^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Delfin J, Martinez I, Antuch W, Morera V, Gonzalez Y, Rodriguez R, Marquez M, Saroyan A, Larionova N, Diaz J, Padron G, Chavez M. Purification, characterization and immobilization of proteinase inhibitors from Stichodactyla helianthus. Toxicon. 1996 Nov-Dec;34(11-12):1367-76. PMID:9027993
↑ Garcia-Fernandez R, Pons T, Perbandt M, Valiente PA, Talavera A, Gonzalez-Gonzalez Y, Rehders D, Chavez MA, Betzel C, Redecke L. Structural insights into serine protease inhibition by a marine invertebrate BPTI Kunitz-type inhibitor. J Struct Biol. 2012 Sep 5. pii: S1047-8477(12)00240-7. doi:, 10.1016/j.jsb.2012.08.009. PMID:22975140 doi:http://dx.doi.org/10.1016/j.jsb.2012.08.009
↑ Garcia-Fernandez R, Pons T, Meyer A, Perbandt M, Gonzalez-Gonzalez Y, Gil D, de los Angeles Chavez M, Betzel C, Redecke L. Structure of the recombinant BPTI/Kunitz-type inhibitor rShPI-1A from the marine invertebrate Stichodactyla helianthus. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Nov 1;68(Pt 11):1289-93., doi: 10.1107/S1744309112039085. Epub 2012 Oct 26. PMID:23143234 doi:http://dx.doi.org/10.1107/S1744309112039085

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3ofw"

@@ Line 1: / Line 1: @@
 ==Crystal structure of recombinant Kunitz Type serine protease Inhibitor-1 from the Carribean sea anemone stichodactyla helianthus==
-<StructureSection load='3ofw' size='340' side='right' caption='[[3ofw]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+<StructureSection load='3ofw' size='340' side='right'caption='[[3ofw]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3ofw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Stihl Stihl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OFW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OFW FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3ofw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Stihl Stihl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OFW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OFW FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3m7q|3m7q]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3m7q|3m7q]]</div></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ofw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ofw OCA], [http://pdbe.org/3ofw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ofw RCSB], [http://www.ebi.ac.uk/pdbsum/3ofw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ofw ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ofw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ofw OCA], [https://pdbe.org/3ofw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ofw RCSB], [https://www.ebi.ac.uk/pdbsum/3ofw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ofw ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/ISH1_STOHE ISH1_STOHE]] Active against serine, cysteine, and aspartic proteinases. Can bind vertebrate trypsin and chymotrypsin.<ref>PMID:9027993</ref> <ref>PMID:22975140</ref>
+[[https://www.uniprot.org/uniprot/ISH1_STOHE ISH1_STOHE]] Active against serine, cysteine, and aspartic proteinases. Can bind vertebrate trypsin and chymotrypsin.<ref>PMID:9027993</ref> <ref>PMID:22975140</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Stihl]]
 [[Category: Betzel, C]]

3ofw

From Proteopedia

Revision as of 10:42, 18 May 2022

Crystal structure of recombinant Kunitz Type serine protease Inhibitor-1 from the Carribean sea anemone stichodactyla helianthus

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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