7ob6
From Proteopedia
(Difference between revisions)
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==CPR-C4 - a conserved novel protease from the Candidate Phyla Radiation== | ==CPR-C4 - a conserved novel protease from the Candidate Phyla Radiation== | ||
| - | <StructureSection load='7ob6' size='340' side='right'caption='[[7ob6]]' scene=''> | + | <StructureSection load='7ob6' size='340' side='right'caption='[[7ob6]], [[Resolution|resolution]] 2.60Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OB6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OB6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7ob6]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OB6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OB6 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ob6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ob6 OCA], [https://pdbe.org/7ob6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ob6 RCSB], [https://www.ebi.ac.uk/pdbsum/7ob6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ob6 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ob6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ob6 OCA], [https://pdbe.org/7ob6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ob6 RCSB], [https://www.ebi.ac.uk/pdbsum/7ob6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ob6 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The Candidate Phyla Radiation is a recently uncovered and vast expansion of the bacterial domain of life, made up of largely uncharacterized phyla that lack isolated representatives. This unexplored territory of genetic diversity presents an abundance of novel proteins with potential applications in the life-science sectors. Here we present the structural and functional elucidation of CPR-C4, a hypothetical protein from the genome of a thermophilic Candidate Phyla Radiation organism, identified through metagenomic sequencing. Our analyses revealed that CPR-C4 is a member of a family of highly conserved proteins within the Candidate Phyla Radiation. The function of CPR-C4 as a cysteine protease was predicted through remote structural similarity to the Homo sapiens vasohibins and subsequently confirmed experimentally with fluorescence-based activity assays. Furthermore, detailed structural and sequence alignment analysis enabled identification of a non-canonical cysteine-histidine-leucine(carbonyl) catalytic triad. The unexpected structural and functional similarities between CPR-C4 and the human vasohibins suggest an evolutionary relationship undetectable at the sequence level alone. | ||
| + | |||
| + | CPR-C4 is a highly conserved novel protease from the Candidate Phyla Radiation with remote structural homology to human vasohibins.,Cornish KAS, Lange J, Aevarsson A, Pohl E J Biol Chem. 2022 Apr 8:101919. doi: 10.1016/j.jbc.2022.101919. PMID:35405098<ref>PMID:35405098</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7ob6" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Cornish | + | [[Category: Cornish, K A.S]] |
| - | [[Category: Pohl E]] | + | [[Category: Pohl, E]] |
| + | [[Category: Cysteine protease]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Protease]] | ||
| + | [[Category: Zn]] | ||
Revision as of 08:12, 25 May 2022
CPR-C4 - a conserved novel protease from the Candidate Phyla Radiation
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