3qj0
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==Crystal Structure of BoNT/A LC complexed with Hydroxamate-based Inhibitor PT-3== | ==Crystal Structure of BoNT/A LC complexed with Hydroxamate-based Inhibitor PT-3== | ||
| - | <StructureSection load='3qj0' size='340' side='right' caption='[[3qj0]], [[Resolution|resolution]] 2.30Å' scene=''> | + | <StructureSection load='3qj0' size='340' side='right'caption='[[3qj0]], [[Resolution|resolution]] 2.30Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[3qj0]] is a 1 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[3qj0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clobh Clobh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QJ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QJ0 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=QI3:(4R)-4-(4-CHLOROPHENOXY)-1-[(4-CHLOROPHENYL)SULFONYL]-N-HYDROXY-L-PROLINAMIDE'>QI3</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=QI3:(4R)-4-(4-CHLOROPHENOXY)-1-[(4-CHLOROPHENYL)SULFONYL]-N-HYDROXY-L-PROLINAMIDE'>QI3</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3qix|3qix]], [[3qiy|3qiy]], [[3qiz|3qiz]]</td></tr> | + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3qix|3qix]], [[3qiy|3qiy]], [[3qiz|3qiz]]</div></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">botA, CBO0806, CLC_0862, Neurotoxin Light Chain ([ | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">botA, CBO0806, CLC_0862, Neurotoxin Light Chain ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=441771 CLOBH])</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qj0 OCA], [https://pdbe.org/3qj0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qj0 RCSB], [https://www.ebi.ac.uk/pdbsum/3qj0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qj0 ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [[ | + | [[https://www.uniprot.org/uniprot/BXA1_CLOBH BXA1_CLOBH]] Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure. |
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 23: | Line 23: | ||
==See Also== | ==See Also== | ||
| - | *[[Botulinum neurotoxin|Botulinum neurotoxin]] | + | *[[Botulinum neurotoxin 3D structures|Botulinum neurotoxin 3D structures]] |
== References == | == References == | ||
<references/> | <references/> | ||
| Line 30: | Line 30: | ||
[[Category: Bontoxilysin]] | [[Category: Bontoxilysin]] | ||
[[Category: Clobh]] | [[Category: Clobh]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Han, G W]] | [[Category: Han, G W]] | ||
[[Category: Stevens, R C]] | [[Category: Stevens, R C]] | ||
Revision as of 06:10, 8 June 2022
Crystal Structure of BoNT/A LC complexed with Hydroxamate-based Inhibitor PT-3
| |||||||||||
