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| | ==Crystal structure of archaeal Spt4/5 bound to the RNAP clamp domain== | | ==Crystal structure of archaeal Spt4/5 bound to the RNAP clamp domain== |
| - | <StructureSection load='3qqc' size='340' side='right' caption='[[3qqc]], [[Resolution|resolution]] 3.30Å' scene=''> | + | <StructureSection load='3qqc' size='340' side='right'caption='[[3qqc]], [[Resolution|resolution]] 3.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3qqc]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrfu Pyrfu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QQC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QQC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3qqc]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrfu Pyrfu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QQC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QQC FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PF1563 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=186497 PYRFU]), PF1990 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=186497 PYRFU]), PF0255 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=186497 PYRFU])</td></tr> | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PF1563 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=186497 PYRFU]), PF1990 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=186497 PYRFU]), PF0255 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=186497 PYRFU])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qqc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qqc OCA], [http://pdbe.org/3qqc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3qqc RCSB], [http://www.ebi.ac.uk/pdbsum/3qqc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3qqc ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qqc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qqc OCA], [https://pdbe.org/3qqc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qqc RCSB], [https://www.ebi.ac.uk/pdbsum/3qqc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qqc ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SPT4_PYRFU SPT4_PYRFU]] Stimulates transcription elongation.<ref>PMID:21386817</ref> <ref>PMID:21187417</ref> [[http://www.uniprot.org/uniprot/SPT5_PYRFU SPT5_PYRFU]] Stimulates transcription elongation.<ref>PMID:21386817</ref> <ref>PMID:21187417</ref> | + | [[https://www.uniprot.org/uniprot/SPT4_PYRFU SPT4_PYRFU]] Stimulates transcription elongation.<ref>PMID:21386817</ref> <ref>PMID:21187417</ref> [[https://www.uniprot.org/uniprot/SPT5_PYRFU SPT5_PYRFU]] Stimulates transcription elongation.<ref>PMID:21386817</ref> <ref>PMID:21187417</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[RNA polymerase|RNA polymerase]] | + | *[[RNA polymerase 3D structures|RNA polymerase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Pyrfu]] | | [[Category: Pyrfu]] |
| | [[Category: Martinez-Rucobo, F W]] | | [[Category: Martinez-Rucobo, F W]] |
| Structural highlights
Function
[SPT4_PYRFU] Stimulates transcription elongation.[1] [2] [SPT5_PYRFU] Stimulates transcription elongation.[3] [4]
Publication Abstract from PubMed
Related RNA polymerases (RNAPs) carry out cellular gene transcription in all three kingdoms of life. The universal conservation of the transcription machinery extends to a single RNAP-associated factor, Spt5 (or NusG in bacteria), which renders RNAP processive and may have arisen early to permit evolution of long genes. Spt5 associates with Spt4 to form the Spt4/5 heterodimer. Here, we present the crystal structure of archaeal Spt4/5 bound to the RNAP clamp domain, which forms one side of the RNAP active centre cleft. The structure revealed a conserved Spt5-RNAP interface and enabled modelling of complexes of Spt4/5 counterparts with RNAPs from all kingdoms of life, and of the complete yeast RNAP II elongation complex with bound Spt4/5. The N-terminal NGN domain of Spt5/NusG closes the RNAP active centre cleft to lock nucleic acids and render the elongation complex stable and processive. The C-terminal KOW1 domain is mobile, but its location is restricted to a region between the RNAP clamp and wall above the RNA exit tunnel, where it may interact with RNA and/or other factors.
Architecture of the RNA polymerase-Spt4/5 complex and basis of universal transcription processivity.,Martinez-Rucobo FW, Sainsbury S, Cheung AC, Cramer P EMBO J. 2011 Mar 8. PMID:21386817[5]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Martinez-Rucobo FW, Sainsbury S, Cheung AC, Cramer P. Architecture of the RNA polymerase-Spt4/5 complex and basis of universal transcription processivity. EMBO J. 2011 Mar 8. PMID:21386817 doi:10.1038/emboj.2011.64
- ↑ Klein BJ, Bose D, Baker KJ, Yusoff ZM, Zhang X, Murakami KS. RNA polymerase and transcription elongation factor Spt4/5 complex structure. Proc Natl Acad Sci U S A. 2011 Jan 11;108(2):546-50. Epub 2010 Dec 27. PMID:21187417 doi:10.1073/pnas.1013828108
- ↑ Martinez-Rucobo FW, Sainsbury S, Cheung AC, Cramer P. Architecture of the RNA polymerase-Spt4/5 complex and basis of universal transcription processivity. EMBO J. 2011 Mar 8. PMID:21386817 doi:10.1038/emboj.2011.64
- ↑ Klein BJ, Bose D, Baker KJ, Yusoff ZM, Zhang X, Murakami KS. RNA polymerase and transcription elongation factor Spt4/5 complex structure. Proc Natl Acad Sci U S A. 2011 Jan 11;108(2):546-50. Epub 2010 Dec 27. PMID:21187417 doi:10.1073/pnas.1013828108
- ↑ Martinez-Rucobo FW, Sainsbury S, Cheung AC, Cramer P. Architecture of the RNA polymerase-Spt4/5 complex and basis of universal transcription processivity. EMBO J. 2011 Mar 8. PMID:21386817 doi:10.1038/emboj.2011.64
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