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3qtn

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Revision as of 05:35, 15 June 2022

Structure of S. pombe nuclear import adaptor Nro1 (Space group P6522)

Structural highlights

3qtn is a 1 chain structure with sequence from Cbs 356. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	3qtm
Gene:	SPCC4B3.07 (CBS 356)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ETT1_SCHPO] Required for correct translation termination (By similarity). Positive regulator of the stability of the N-terminal transcription factor domain (Sre1N) of sre1 which is released from the membrane and enters the nucleus to activate hypoxic gene expression. Acts also as a direct inhibitor of ofd1. Functions probably by inhibiting the ability of the ofd1 to accelerate Sre1N degradation in absence of oxygen.^[1] ^[2]

Publication Abstract from PubMed

In Saccharomyces cerevisiae, the putative 2-OG-Fe(II) dioxygenase Tpa1 and its partner Ett1 have been shown to impact mRNA decay and translation. Hence, inactivation of these factors was shown to influence stop codon read-though. In addition, Tpa1 represses, by an unknown mechanism, genes regulated by Hap1, a transcription factor involved in the response to levels of heme and O(2). The Schizosaccharomyces pombe orthologs of Tpa1 and Ett1, Ofd1, and its partner Nro1, respectively, have been shown to regulate the stability of the Sre1 transcription factor in response to oxygen levels. To gain insight into the function of Nro1/Ett1, we have solved the crystal structure of the S. pombe Nro1 protein deleted of its 54 N-terminal residues. Nro1 unexpectedly adopts a Tetratrico Peptide Repeat (TPR) fold, a motif often responsible for protein or peptide binding. Two ligands, a sulfate ion and an unknown molecule, interact with a cluster of highly conserved amino acids on the protein surface. Mutation of these residues demonstrates that these ligand binding sites are essential for Ett1 function in S. cerevisiae, as investigated by assaying for efficient translation termination.

Structural and functional analysis of Nro1/Ett1: a protein involved in translation termination in S. cerevisiae and in O2-mediated gene control in S. pombe.,Rispal D, Henri J, van Tilbeurgh H, Graille M, Seraphin B RNA. 2011 Jul;17(7):1213-24. Epub 2011 May 24. PMID:21610214^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chen XQ, Du X, Liu J, Balasubramanian MK, Balasundaram D. Identification of genes encoding putative nucleoporins and transport factors in the fission yeast Schizosaccharomyces pombe: a deletion analysis. Yeast. 2004 Apr 30;21(6):495-509. PMID:15116432 doi:http://dx.doi.org/10.1002/yea.1115
↑ Lee CY, Stewart EV, Hughes BT, Espenshade PJ. Oxygen-dependent binding of Nro1 to the prolyl hydroxylase Ofd1 regulates SREBP degradation in yeast. EMBO J. 2009 Jan 21;28(2):135-43. doi: 10.1038/emboj.2008.271. PMID:19158663 doi:http://dx.doi.org/10.1038/emboj.2008.271
↑ Rispal D, Henri J, van Tilbeurgh H, Graille M, Seraphin B. Structural and functional analysis of Nro1/Ett1: a protein involved in translation termination in S. cerevisiae and in O2-mediated gene control in S. pombe. RNA. 2011 Jul;17(7):1213-24. Epub 2011 May 24. PMID:21610214 doi:10.1261/rna.2697111

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3qtn"

@@ Line 1: / Line 1: @@
 ==Structure of S. pombe nuclear import adaptor Nro1 (Space group P6522)==
-<StructureSection load='3qtn' size='340' side='right' caption='[[3qtn]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
+<StructureSection load='3qtn' size='340' side='right'caption='[[3qtn]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3qtn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cbs_356 Cbs 356]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QTN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QTN FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3qtn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cbs_356 Cbs 356]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QTN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QTN FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3qtm|3qtm]]</td></tr>
+</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3qtm|3qtm]]</div></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SPCC4B3.07 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 CBS 356])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SPCC4B3.07 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 CBS 356])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qtn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qtn OCA], [http://pdbe.org/3qtn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3qtn RCSB], [http://www.ebi.ac.uk/pdbsum/3qtn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3qtn ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qtn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qtn OCA], [https://pdbe.org/3qtn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qtn RCSB], [https://www.ebi.ac.uk/pdbsum/3qtn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qtn ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/ETT1_SCHPO ETT1_SCHPO]] Required for correct translation termination (By similarity). Positive regulator of the stability of the N-terminal transcription factor domain (Sre1N) of sre1 which is released from the membrane and enters the nucleus to activate hypoxic gene expression. Acts also as a direct inhibitor of ofd1. Functions probably by inhibiting the ability of the ofd1 to accelerate Sre1N degradation in absence of oxygen.<ref>PMID:15116432</ref> <ref>PMID:19158663</ref>
+[[https://www.uniprot.org/uniprot/ETT1_SCHPO ETT1_SCHPO]] Required for correct translation termination (By similarity). Positive regulator of the stability of the N-terminal transcription factor domain (Sre1N) of sre1 which is released from the membrane and enters the nucleus to activate hypoxic gene expression. Acts also as a direct inhibitor of ofd1. Functions probably by inhibiting the ability of the ofd1 to accelerate Sre1N degradation in absence of oxygen.<ref>PMID:15116432</ref> <ref>PMID:19158663</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 24: @@
 </StructureSection>
 [[Category: Cbs 356]]
+[[Category: Large Structures]]
 [[Category: Graille, M]]
 [[Category: Henri, J]]

3qtn

From Proteopedia

Revision as of 05:35, 15 June 2022

Structure of S. pombe nuclear import adaptor Nro1 (Space group P6522)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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