User:Arthur Migliatti/Sandbox1

Thioredoxin(Trx) is a protein present in all organisms, from bacterias to complex beings as humans. This page will be focused on exploring the characteristics of Trx1, a cytosolic form of Trx present in eukaryotes. Trx1 has an active site composed of 2 cysteines separated by 2 aminoacids () which catalyses the reduction of other thiol-proteins and becomes oxidized. It is reduced back by Thioredoxin Reductase(TrxR), which, in the end, is reduced by NADPH. Together, the two proteins and NADPH form the system Trx^[1]. As it is shown in this , both Cys32 and Cys 35 were highly conserved during evolution(dark pink). One of the most important proteins that Trx reduces is Peroxiredoxin(Prx), which catalyses the reduction of Hidrogen Peroxide(H2O2) to water. Since high concentrations of H2O2 produces other potent oxidizing molecules, such as hydroxyl radical, Prx's action, and so Trx's also, are fundamental for the cell to have a redox homeostasis and to have low amount of damage.

To reduce other proteins, first happens an attack from Cys32, creating an intermolecular dissulfide bond, represented between residue Cys32 from Trx1 and residue Cys206 from MsrA. After it, residue Cys35 attacks Cys32, creating a dissulfide bond between the two cysteines in Trx1's catalytic site. This is the .

Structure

Trx1 is a monomeric protein and weights around 12kDa. It is formed by one five-stranded beta sheets involved by 4 alpha helix, shown . The active site is located on a lump between betra strand 2, where Cys 35 is located, and alpha helix 2, where Cys32 is located. By being in the end of an alpha helix, has a lower pKa, making it possible to reduce dissulfide bonds.^[2]

Function

Trx and TrxR were first discovered in 1964 in a study realized in bacteria, and were described as necessary proteins to reduce Ribonucleotide Reductase(RNR), a protein that produces deoxyribonucleotides from ribonucleotides^[3].

Since 1964, other functions of Trx1, different than participating in cell division, were discovered, as denitrosation and transnitrosation for example. Denitrosation is the removal of NO of a protein, and Trx1 does it by being temporarily S-nitrosataded on Cys32. Aterwards, Cys35 attacks Cys32 and forms a dissulfide bond, releasing HNO/NO to the medium. On the other hand, transnitrosation is the the nitrosation of other proteins, that the Trx1 of some species can do.

Although Trx1 from a great amount of organisms has only the catalytic site cysteines, the human form of Trx1 also has other , Cys 62, Cys 69 and Cys 73, which can act as regulators of the protein. enhances its antiapoptotic function in some cases, although its not necessary for it.^[4]. Cys 73 has more than one function. Firstly, it is through this residue that Trx1 transnitrosate other proteins, the Trx of not all organisms are capable of doing transnitrosation. Another function is to make Trx1 a sensor of the redox state of the cell. When the cell is in a strong oxidizing state, Trx1 forms an homodimer connected by a . Since Cys73 is spacially close to the active site, the formation of a dimer prevents Trx1 from interacting with other proteins and reducing them (black = residues Cys73, pink = active site from monomer A, orange = active site from monomer B).

Falar da RNR, Prx e outras proteínas que eu tenho visto que ela interage.

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