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1hji
From Proteopedia
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[[Image:1hji.gif|left|200px]] | [[Image:1hji.gif|left|200px]] | ||
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| - | + | {{STRUCTURE_1hji| PDB=1hji | SCENE= }} | |
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'''BACTERIOPHAGE HK022 NUN-PROTEIN-NUTBOXB-RNA COMPLEX''' | '''BACTERIOPHAGE HK022 NUN-PROTEIN-NUTBOXB-RNA COMPLEX''' | ||
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[[Category: Schaerpf, M.]] | [[Category: Schaerpf, M.]] | ||
[[Category: Sticht, H.]] | [[Category: Sticht, H.]] | ||
| - | [[Category: | + | [[Category: Bacteriophage hk022]] |
| - | [[Category: | + | [[Category: Peptide-rna-complex]] |
| - | [[Category: | + | [[Category: Peptide-rna-recognition]] |
| - | [[Category: | + | [[Category: Protein/rna]] |
| - | [[Category: | + | [[Category: Termination]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:54:38 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 15:54, 2 May 2008
BACTERIOPHAGE HK022 NUN-PROTEIN-NUTBOXB-RNA COMPLEX
Overview
Nun protein from coliphage HK022 binds to phage boxB RNA and functions, in contrast to phage lambda N protein, as a transcriptional terminator. The basic Nun-(10-44) peptide contains the boxB RNA binding arginine rich motif, ARM. The peptide binds boxB RNA and competes with the phage lambda ARM peptide N-(1-36) as indicated by nuclear magnetic resonance (NMR) spectroscopy titrations. In two-dimensional nuclear Overhauser enhancement spectroscopy experiments boxB RNA in complex with Nun-(20-44) exhibits the same pattern of resonances as it does in complex with N peptides containing the ARM, and we could show that Nun-(20-44) forms a bent alpha-helix upon binding to the boxB RNA. The structure of the boxB RNA-bound Nun-(20-44) was determined on the basis of 191 intra- and 30 intermolecular distance restraints. Ser-24 is anchored to the lower RNA stem, and stacking of Tyr-39 and A7 is clearly experimentally indicated. Arg-28 shows numerous contacts to the RNA stem. Leu-22, Ile-30, Trp-33, Ile-37, and Leu-41 form a hydrophobic surface, which could be a recognition site for additional host factors such as NusG. Such a hydrophobic surface area is not present in N-(1-36) bound to boxB RNA.
About this Structure
1HJI is a Protein complex structure of sequences from Enterobacteria phage hk022. Full crystallographic information is available from OCA.
Reference
The structure of the coliphage HK022 Nun protein-lambda-phage boxB RNA complex. Implications for the mechanism of transcription termination., Faber C, Scharpf M, Becker T, Sticht H, Rosch P, J Biol Chem. 2001 Aug 24;276(34):32064-70. Epub 2001 May 16. PMID:11356847 Page seeded by OCA on Fri May 2 18:54:38 2008
