1hjk

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[[Image:1hjk.gif|left|200px]]
[[Image:1hjk.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1hjk |SIZE=350|CAPTION= <scene name='initialview01'>1hjk</scene>, resolution 2.3&Aring;
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The line below this paragraph, containing "STRUCTURE_1hjk", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=CTA:Catalytically+Active+Site+On+A+Subunit'>CTA</scene> and <scene name='pdbsite=CTB:Catalytically+Active+Site+On+B+Subunit'>CTB</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= PHOA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-->
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|DOMAIN=
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{{STRUCTURE_1hjk| PDB=1hjk | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hjk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hjk OCA], [http://www.ebi.ac.uk/pdbsum/1hjk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hjk RCSB]</span>
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}}
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'''ALKALINE PHOSPHATASE MUTANT H331Q'''
'''ALKALINE PHOSPHATASE MUTANT H331Q'''
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[[Category: Murphy, J E.]]
[[Category: Murphy, J E.]]
[[Category: Stec, B.]]
[[Category: Stec, B.]]
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[[Category: alcohol acceptor)]]
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[[Category: Alkaline phosphatase]]
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[[Category: alkaline phosphatase]]
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[[Category: Hydrolase]]
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[[Category: hydrolase]]
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[[Category: Phosphoric monoester]]
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[[Category: phosphoric monoester]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:54:46 2008''
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[[Category: transferase(phospho]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:06:08 2008''
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Revision as of 15:54, 2 May 2008

Image:1hjk.gif

Template:STRUCTURE 1hjk

ALKALINE PHOSPHATASE MUTANT H331Q


Overview

Using a mutant version of E. coli alkaline phosphatase, we succeeded in trapping and determining the structure of the phospho-enzyme intermediate. The X-ray structure also revealed the catalytic water molecule, bound to one of the active site zinc ions, positioned ideally for the apical attack necessary for the hydrolysis of the intermediate.

About this Structure

1HJK is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Trapping and visualization of a covalent enzyme-phosphate intermediate., Murphy JE, Stec B, Ma L, Kantrowitz ER, Nat Struct Biol. 1997 Aug;4(8):618-22. PMID:9253408 Page seeded by OCA on Fri May 2 18:54:46 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

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