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3sn8
From Proteopedia
(Difference between revisions)
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==Crystal structure of SARS coronavirus main protease complexed with Cm-FF-H (soaking)== | ==Crystal structure of SARS coronavirus main protease complexed with Cm-FF-H (soaking)== | ||
| - | <StructureSection load='3sn8' size='340' side='right' caption='[[3sn8]], [[Resolution|resolution]] 1.99Å' scene=''> | + | <StructureSection load='3sn8' size='340' side='right'caption='[[3sn8]], [[Resolution|resolution]] 1.99Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[3sn8]] is a 1 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[3sn8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cvhsa Cvhsa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SN8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SN8 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=S89:N-[(2S)-1-HYDROXY-3-PHENYLPROPAN-2-YL]-NALPHA-[(2E)-3-PHENYLPROP-2-ENOYL]-L-PHENYLALANINAMIDE'>S89</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=S89:N-[(2S)-1-HYDROXY-3-PHENYLPROPAN-2-YL]-NALPHA-[(2E)-3-PHENYLPROP-2-ENOYL]-L-PHENYLALANINAMIDE'>S89</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2h2z|2h2z]], [[3sna|3sna]], [[3snb|3snb]], [[3snc|3snc]], [[3snd|3snd]], [[3sne|3sne]]</td></tr> | + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2h2z|2h2z]], [[3sna|3sna]], [[3snb|3snb]], [[3snc|3snc]], [[3snd|3snd]], [[3sne|3sne]]</div></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sn8 OCA], [https://pdbe.org/3sn8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sn8 RCSB], [https://www.ebi.ac.uk/pdbsum/3sn8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sn8 ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [[ | + | [[https://www.uniprot.org/uniprot/R1A_CVHSA R1A_CVHSA]] The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK (By similarity). Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> Nsp9 is a ssRNA-binding protein.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> |
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</div> | </div> | ||
<div class="pdbe-citations 3sn8" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 3sn8" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Virus protease 3D structures|Virus protease 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Cvhsa]] | [[Category: Cvhsa]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Hilgenfeld, R]] | [[Category: Hilgenfeld, R]] | ||
[[Category: Zhu, L]] | [[Category: Zhu, L]] | ||
Current revision
Crystal structure of SARS coronavirus main protease complexed with Cm-FF-H (soaking)
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