1hkv

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[[Image:1hkv.gif|left|200px]]
[[Image:1hkv.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1hkv |SIZE=350|CAPTION= <scene name='initialview01'>1hkv</scene>, resolution 2.60&Aring;
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The line below this paragraph, containing "STRUCTURE_1hkv", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=PLA:LYS+Binding+Site+For+Chain+B'>PLA</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5&#39;-PHOSPHATE'>PLP</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Diaminopimelate_decarboxylase Diaminopimelate decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.20 4.1.1.20] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam00278 Orn_DAP_Arg_deC], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam01168 Ala_racemase_N], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG0019 LysA], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd00635 YBL036c_PLPDEIII]</span>
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{{STRUCTURE_1hkv| PDB=1hkv | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hkv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hkv OCA], [http://www.ebi.ac.uk/pdbsum/1hkv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hkv RCSB]</span>
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}}
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'''MYCOBACTERIUM DIAMINOPIMELATE DICARBOXYLASE (LYSA)'''
'''MYCOBACTERIUM DIAMINOPIMELATE DICARBOXYLASE (LYSA)'''
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[[Category: Sacchettini, J C.]]
[[Category: Sacchettini, J C.]]
[[Category: TBSGC, TB Structural Genomics Consortium.]]
[[Category: TBSGC, TB Structural Genomics Consortium.]]
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[[Category: dapdc]]
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[[Category: Dapdc]]
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[[Category: decarboxylase]]
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[[Category: Decarboxylase]]
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[[Category: diaminopimelate]]
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[[Category: Diaminopimelate]]
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[[Category: lyase]]
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[[Category: Lyase]]
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[[Category: lysine pathway]]
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[[Category: Lysine pathway]]
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[[Category: lysine synthetic pathway,pyridoxal phosphate,psi,protein structure initiative,tb structural genomics consortium,tb]]
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[[Category: Lysine synthetic pathway,pyridoxal phosphate,psi,protein structure initiative,tb structural genomics consortium,tb]]
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[[Category: mycbacterium tuberculosis]]
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[[Category: Mycbacterium tuberculosis]]
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[[Category: plp]]
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[[Category: Plp]]
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[[Category: tbsgc]]
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[[Category: Tbsgc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:57:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:06:53 2008''
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Revision as of 15:57, 2 May 2008

Image:1hkv.gif

Template:STRUCTURE 1hkv

MYCOBACTERIUM DIAMINOPIMELATE DICARBOXYLASE (LYSA)


Overview

The Mycobacterium tuberculosis lysA gene encodes the enzyme meso-diaminopimelate decarboxylase (DAPDC), a pyridoxal-5'-phosphate (PLP)-dependent enzyme. The enzyme catalyzes the final step in the lysine biosynthetic pathway converting meso-diaminopimelic acid (DAP) to l-lysine. The lysA gene of M. tuberculosis H37Rv has been established as essential for bacterial survival in immunocompromised mice, demonstrating that de novo biosynthesis of lysine is essential for in vivo viability. Drugs targeted against DAPDC could be efficient anti-tuberculosis drugs, and the three-dimensional structure of DAPDC from M. tuberculosis complexed with reaction product lysine and the ternary complex with PLP and lysine in the active site has been determined. The first structure of a DAPDC confirms its classification as a fold type III PLP-dependent enzyme. The structure shows a stable 2-fold dimer in head-to-tail arrangement of a triose-phosphate isomerase (TIM) barrel-like alpha/beta domain and a C-terminal beta sheet domain, similar to the ornithine decarboxylase (ODC) fold family. PLP is covalently bound via an internal aldimine, and residues from both domains and both subunits contribute to the binding pocket. Comparison of the structure with eukaryotic ODCs, in particular with a di-fluoromethyl ornithine (DMFO)-bound ODC from Trypanosoma bruceii, indicates that corresponding DAP-analogues might be potential inhibitors for mycobacterial DAPDCs.

About this Structure

1HKV is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

Reference

Crystal structure of Mycobacterium tuberculosis diaminopimelate decarboxylase, an essential enzyme in bacterial lysine biosynthesis., Gokulan K, Rupp B, Pavelka MS Jr, Jacobs WR Jr, Sacchettini JC, J Biol Chem. 2003 May 16;278(20):18588-96. Epub 2003 Mar 10. PMID:12637582 Page seeded by OCA on Fri May 2 18:57:37 2008

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