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3sze

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Revision as of 08:17, 29 June 2022

Crystal structure of the passenger domain of the E. coli autotransporter EspP

Structural highlights

3sze is a 1 chain structure with sequence from Escherichia coli 0157:h7 edl933. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	ECO57PM78, espP, L7020 (Escherichia coli 0157:H7 EDL933)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ESPP_ECO57] Serine protease capable of cleaving pepsin A and human coagulation factor V, which may contribute to the mucosal hemorrhage observed in hemorrhagic colitis.^[1] ^[2]

Publication Abstract from PubMed

Autotransporters represent a large superfamily of known and putative virulence factors produced by Gram-negative bacteria. They consist of an N-terminal "passenger domain" responsible for the specific effector functions of the molecule and a C-terminal "beta-domain" responsible for translocation of the passenger across the bacterial outer membrane. Here, we present the 2.5-A crystal structure of the passenger domain of the extracellular serine protease EspP, produced by the pathogen Escherichia coli O157:H7 and a member of the serine protease autotransporters of Enterobacteriaceae (SPATEs). Like the previously structurally characterized SPATE passenger domains, the EspP passenger domain contains an extended right-handed parallel beta-helix preceded by an N-terminal globular domain housing the catalytic function of the protease. Of note, however, is the absence of a second globular domain protruding from this beta-helix. We describe the structure of the EspP passenger domain in the context of previous results and provide an alternative hypothesis for the function of the beta-helix within SPATEs.

Crystal Structure of the Passenger Domain of the Escherichia coli Autotransporter EspP.,Khan S, Mian HS, Sandercock LE, Chirgadze NY, Pai EF J Mol Biol. 2011 Sep 22. PMID:21964244^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Brunder W, Schmidt H, Karch H. EspP, a novel extracellular serine protease of enterohaemorrhagic Escherichia coli O157:H7 cleaves human coagulation factor V. Mol Microbiol. 1997 May;24(4):767-78. PMID:9194704
↑ Szabady RL, Peterson JH, Skillman KM, Bernstein HD. An unusual signal peptide facilitates late steps in the biogenesis of a bacterial autotransporter. Proc Natl Acad Sci U S A. 2005 Jan 4;102(1):221-6. Epub 2004 Dec 22. PMID:15615856 doi:http://dx.doi.org/10.1073/pnas.0406055102
↑ Khan S, Mian HS, Sandercock LE, Chirgadze NY, Pai EF. Crystal Structure of the Passenger Domain of the Escherichia coli Autotransporter EspP. J Mol Biol. 2011 Sep 22. PMID:21964244 doi:10.1016/j.jmb.2011.09.028

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3sze"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the passenger domain of the E. coli autotransporter EspP==
-<StructureSection load='3sze' size='340' side='right' caption='[[3sze]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+<StructureSection load='3sze' size='340' side='right'caption='[[3sze]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3sze]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_0157:h7_edl933 Escherichia coli 0157:h7 edl933]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SZE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SZE FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3sze]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_0157:h7_edl933 Escherichia coli 0157:h7 edl933]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SZE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SZE FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ECO57PM78, espP, L7020 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=155864 Escherichia coli 0157:H7 EDL933])</td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ECO57PM78, espP, L7020 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=155864 Escherichia coli 0157:H7 EDL933])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sze OCA], [http://pdbe.org/3sze PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3sze RCSB], [http://www.ebi.ac.uk/pdbsum/3sze PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3sze ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sze OCA], [https://pdbe.org/3sze PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sze RCSB], [https://www.ebi.ac.uk/pdbsum/3sze PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sze ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/ESPP_ECO57 ESPP_ECO57]] Serine protease capable of cleaving pepsin A and human coagulation factor V, which may contribute to the mucosal hemorrhage observed in hemorrhagic colitis.<ref>PMID:9194704</ref> <ref>PMID:15615856</ref>
+[[https://www.uniprot.org/uniprot/ESPP_ECO57 ESPP_ECO57]] Serine protease capable of cleaving pepsin A and human coagulation factor V, which may contribute to the mucosal hemorrhage observed in hemorrhagic colitis.<ref>PMID:9194704</ref> <ref>PMID:15615856</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 </StructureSection>
 [[Category: Escherichia coli 0157:h7 edl933]]
+[[Category: Large Structures]]
 [[Category: Battaile, K P]]
 [[Category: Chirgadze, N Y]]

3sze

From Proteopedia

Revision as of 08:17, 29 June 2022

Crystal structure of the passenger domain of the E. coli autotransporter EspP

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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