1hlb
From Proteopedia
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[[Category: Hackert, M L.]] | [[Category: Hackert, M L.]] | ||
[[Category: Mitchell, D T.]] | [[Category: Mitchell, D T.]] | ||
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Revision as of 15:58, 2 May 2008
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Overview
The X-ray structures of two hemoglobins (Hb) from the sea cucumber Caudina arenicola (an echinoderm) have been determined: a low spin, hemichrome, monomeric Hb-C chain, and a cyanomet-liganded dimeric Hb-D chain. Attempts to obtain crystal structures of the deoxy-liganded and hemichrome forms from the same chain type have not been successful. In this work, the Hb-C chain and Hb-D chain structures are compared, and differences observed in tertiary structure related to the different ligand states for hemoglobin chains from this organism. In addition to shifts of the distal histidine and E helix, differences are noted in the position of the heme group within the heme pocket, the hydrogen bonding of the heme group to the protein, and the status of the D helix. These differences are important in understanding the ligand-linked association states of these hemoglobins. The quaternary structure of the Hb-D homodimer is compared with those from two other invertebrate hemoglobins from Scapharca inaequivalvis and Urechis caupo, which also have subunit-subunit interactions that involve the E and E' helices. The dimer interactions of the Caudina and Urechis hemoglobins are quite dissimilar. However, the dimer interface observed in cyanomet Hb-D is strikingly similar to that observed for the carbonmonoxy hemoglobin dimer from the clam, Scapharca, yet many of the key amino acid residues implicated in the cooperative mechanism of the Scapharca hemoglobin are not conserved in the Caudina hemoglobins.
About this Structure
1HLB is a Single protein structure of sequence from Caudina arenicola. Full crystallographic information is available from OCA.
Reference
Structural analysis of monomeric hemichrome and dimeric cyanomet hemoglobins from Caudina arenicola., Mitchell DT, Kitto GB, Hackert ML, J Mol Biol. 1995 Aug 18;251(3):421-31. PMID:7650740 Page seeded by OCA on Fri May 2 18:58:38 2008
