7o43
From Proteopedia
(Difference between revisions)
Line 1: | Line 1: | ||
==TrwK/VirB4unbound dimer complex from R388 type IV secretion system determined by cryo-EM.== | ==TrwK/VirB4unbound dimer complex from R388 type IV secretion system determined by cryo-EM.== | ||
- | <StructureSection load='7o43' size='340' side='right'caption='[[7o43]]' scene=''> | + | <StructureSection load='7o43' size='340' side='right'caption='[[7o43]], [[Resolution|resolution]] 3.40Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
- | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O43 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O43 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7o43]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O43 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O43 FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o43 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o43 OCA], [https://pdbe.org/7o43 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o43 RCSB], [https://www.ebi.ac.uk/pdbsum/7o43 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o43 ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o43 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o43 OCA], [https://pdbe.org/7o43 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o43 RCSB], [https://www.ebi.ac.uk/pdbsum/7o43 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o43 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
+ | <div style="background-color:#fffaf0;"> | ||
+ | == Publication Abstract from PubMed == | ||
+ | Bacterial conjugation is the fundamental process of unidirectional transfer of DNAs, often plasmid DNAs, from a donor cell to a recipient cell(1). It is the primary means by which antibiotic resistance genes spread among bacterial populations(2,3). In Gram-negative bacteria, conjugation is mediated by a large transport apparatus-the conjugative type IV secretion system (T4SS)-produced by the donor cell and embedded in both its outer and inner membranes. The T4SS also elaborates a long extracellular filament-the conjugative pilus-that is essential for DNA transfer(4,5). Here we present a high-resolution cryo-electron microscopy (cryo-EM) structure of a 2.8 megadalton T4SS complex composed of 92 polypeptides representing 8 of the 10 essential T4SS components involved in pilus biogenesis. We added the two remaining components to the structural model using co-evolution analysis of protein interfaces, to enable the reconstitution of the entire system including the pilus. This structure describes the exceptionally large protein-protein interaction network required to assemble the many components that constitute a T4SS and provides insights on the unique mechanism by which they elaborate pili. | ||
+ | |||
+ | Cryo-EM structure of a type IV secretion system.,Mace K, Vadakkepat AK, Redzej A, Lukoyanova N, Oomen C, Braun N, Ukleja M, Lu F, Costa TRD, Orlova EV, Baker D, Cong Q, Waksman G Nature. 2022 Jun 22. pii: 10.1038/s41586-022-04859-y. doi:, 10.1038/s41586-022-04859-y. PMID:35732732<ref>PMID:35732732</ref> | ||
+ | |||
+ | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
+ | </div> | ||
+ | <div class="pdbe-citations 7o43" style="background-color:#fffaf0;"></div> | ||
+ | == References == | ||
+ | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
- | [[Category: Lukoyanova N]] | + | [[Category: Lukoyanova, N]] |
- | [[Category: Mace K]] | + | [[Category: Mace, K]] |
- | [[Category: Vadakkepat | + | [[Category: Vadakkepat, A K]] |
- | [[Category: Waksman G]] | + | [[Category: Waksman, G]] |
+ | [[Category: Atpase]] | ||
+ | [[Category: Bacterial secretion]] | ||
+ | [[Category: Conjugation]] | ||
+ | [[Category: Dimer]] | ||
+ | [[Category: Hcp]] | ||
+ | [[Category: Inner membrane]] | ||
+ | [[Category: Inner membrane complex]] | ||
+ | [[Category: Membrane protein]] | ||
+ | [[Category: Protein complex]] | ||
+ | [[Category: R388 plasmid]] | ||
+ | [[Category: Secretion]] | ||
+ | [[Category: Secretion system]] | ||
+ | [[Category: T4ss]] | ||
+ | [[Category: Trwk]] | ||
+ | [[Category: Type 4 secretion system]] | ||
+ | [[Category: Type iv secretion system]] | ||
+ | [[Category: Virb4]] |
Revision as of 16:22, 6 July 2022
TrwK/VirB4unbound dimer complex from R388 type IV secretion system determined by cryo-EM.
|
Categories: Large Structures | Lukoyanova, N | Mace, K | Vadakkepat, A K | Waksman, G | Atpase | Bacterial secretion | Conjugation | Dimer | Hcp | Inner membrane | Inner membrane complex | Membrane protein | Protein complex | R388 plasmid | Secretion | Secretion system | T4ss | Trwk | Type 4 secretion system | Type iv secretion system | Virb4