1hm6
From Proteopedia
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'''X-RAY STRUCTURE OF FULL-LENGTH ANNEXIN 1''' | '''X-RAY STRUCTURE OF FULL-LENGTH ANNEXIN 1''' | ||
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[[Category: Luecke, H.]] | [[Category: Luecke, H.]] | ||
[[Category: Rosengarth, A.]] | [[Category: Rosengarth, A.]] | ||
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| - | [[Category: | + | [[Category: Full-length protein comprising protein core and n-terminal domain]] |
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Revision as of 16:00, 2 May 2008
X-RAY STRUCTURE OF FULL-LENGTH ANNEXIN 1
Overview
Annexins comprise a multigene family of Ca2+ and phospholipid- binding proteins. They consist of a conserved C-terminal or core domain that confers Ca2+-dependent phospholipid binding and an N-terminal domain that is variable in sequence and length and responsible for the specific properties of each annexin. Crystal structures of various annexin core domains have revealed a high degree of similarity. From these and other studies it is evident that the core domain harbors the calcium-binding sites that interact with the phospholipid headgroups. However, no structure has been reported of an annexin with a complete N-terminal domain. We have now solved the crystal structure of such a full-length annexin, annexin 1. Annexin 1 is active in membrane aggregation and its refined 1.8 A structure shows an alpha-helical N-terminal domain connected to the core domain by a flexible linker. It is surprising that the two alpha-helices present in the N-terminal domain of 41 residues interact intimately with the core domain, with the amphipathic helix 2-12 of the N-terminal domain replacing helix D of repeat III of the core. In turn, helix D is unwound into a flap now partially covering the N-terminal helix. Implications for membrane aggregation will be discussed and a model of aggregation based on the structure will be presented.
About this Structure
1HM6 is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.
Reference
X-ray structure of full-length annexin 1 and implications for membrane aggregation., Rosengarth A, Gerke V, Luecke H, J Mol Biol. 2001 Feb 23;306(3):489-98. PMID:11178908 Page seeded by OCA on Fri May 2 19:00:23 2008
