|
|
| Line 1: |
Line 1: |
| | | | |
| | ==Crystal Structure of the zinc-dependent MarR Family Transcriptional Regulator AdcR in the Zn(II)-bound State== | | ==Crystal Structure of the zinc-dependent MarR Family Transcriptional Regulator AdcR in the Zn(II)-bound State== |
| - | <StructureSection load='3tgn' size='340' side='right' caption='[[3tgn]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='3tgn' size='340' side='right'caption='[[3tgn]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3tgn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Strp2 Strp2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TGN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TGN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3tgn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Strp2 Strp2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TGN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TGN FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">adcR, SPD_2000 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=373153 STRP2])</td></tr> | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">adcR, SPD_2000 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=373153 STRP2])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tgn OCA], [http://pdbe.org/3tgn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tgn RCSB], [http://www.ebi.ac.uk/pdbsum/3tgn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tgn ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tgn OCA], [https://pdbe.org/3tgn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tgn RCSB], [https://www.ebi.ac.uk/pdbsum/3tgn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tgn ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ADCR_STRP2 ADCR_STRP2]] Zinc-responsive regulator that acts both as a repressor and as an activator by regulating directly the promoters of its target genes. In the presence of zinc, directly represses the expression of the adcRCBA operon, of genes coding for a group of surface antigen zinc-binding pneumococcal histidine triad proteins (PhtA, PhtB, PhtD and PhtE), and of adcAII. Can also activate expression of adh.<ref>PMID:20804771</ref> <ref>PMID:21603707</ref> | + | [[https://www.uniprot.org/uniprot/ADCR_STRP2 ADCR_STRP2]] Zinc-responsive regulator that acts both as a repressor and as an activator by regulating directly the promoters of its target genes. In the presence of zinc, directly represses the expression of the adcRCBA operon, of genes coding for a group of surface antigen zinc-binding pneumococcal histidine triad proteins (PhtA, PhtB, PhtD and PhtE), and of adcAII. Can also activate expression of adh.<ref>PMID:20804771</ref> <ref>PMID:21603707</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 23: |
Line 23: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Strp2]] | | [[Category: Strp2]] |
| | [[Category: Giedroc, D P]] | | [[Category: Giedroc, D P]] |
| Structural highlights
Function
[ADCR_STRP2] Zinc-responsive regulator that acts both as a repressor and as an activator by regulating directly the promoters of its target genes. In the presence of zinc, directly represses the expression of the adcRCBA operon, of genes coding for a group of surface antigen zinc-binding pneumococcal histidine triad proteins (PhtA, PhtB, PhtD and PhtE), and of adcAII. Can also activate expression of adh.[1] [2]
Publication Abstract from PubMed
Streptococcus pneumoniae adhesin competence regulator (AdcR), the first metal-dependent member of the multiple antibiotic resistance regulator (MarR) family of proteins, represses the transcription of a high-affinity zinc-specific uptake transporter, a group of surface antigen zinc-binding pneumococcal histidine triad proteins (PhtA, PhtB, PhtD, and PhtE), and an AdcA homologue (AdcAII). The 2.0 A resolution structure of Zn(II)-bound AdcR reveals a highly helical two-fold-symmetric dimer with two distinct metal-binding sites per protomer. Zn(II) is tetrahedrally coordinated by E24, H42, H108, and H112 in what defines the primary sensing site in AdcR. Site 2 is a tetracoordinate site whose function is currently unknown. NMR methyl group perturbation experiments reveal that Zn(II) drives a global change in the structure of apo-AdcR that stabilizes a conformation that is compatible with DNA binding. This co-repression mechanism is unprecedented in MarR transcriptional regulators.
Crystal Structure of the Zinc-Dependent MarR Family Transcriptional Regulator AdcR in the Zn(II)-Bound State.,Guerra AJ, Dann CE, Giedroc DP J Am Chem Soc. 2011 Nov 21. PMID:22085181[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Reyes-Caballero H, Guerra AJ, Jacobsen FE, Kazmierczak KM, Cowart D, Koppolu UM, Scott RA, Winkler ME, Giedroc DP. The metalloregulatory zinc site in Streptococcus pneumoniae AdcR, a zinc-activated MarR family repressor. J Mol Biol. 2010 Oct 22;403(2):197-216. doi: 10.1016/j.jmb.2010.08.030. Epub 2010, Sep 8. PMID:20804771 doi:http://dx.doi.org/10.1016/j.jmb.2010.08.030
- ↑ Shafeeq S, Kloosterman TG, Kuipers OP. Transcriptional response of Streptococcus pneumoniae to Zn2+) limitation and the repressor/activator function of AdcR. Metallomics. 2011 Jun;3(6):609-18. doi: 10.1039/c1mt00030f. Epub 2011 May 21. PMID:21603707 doi:http://dx.doi.org/10.1039/c1mt00030f
- ↑ Guerra AJ, Dann CE, Giedroc DP. Crystal Structure of the Zinc-Dependent MarR Family Transcriptional Regulator AdcR in the Zn(II)-Bound State. J Am Chem Soc. 2011 Nov 21. PMID:22085181 doi:10.1021/ja2080532
|
