1ho0
From Proteopedia
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[[Image:1ho0.jpg|left|200px]] | [[Image:1ho0.jpg|left|200px]] | ||
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'''NEW B-CHAIN MUTANT OF BOVINE INSULIN''' | '''NEW B-CHAIN MUTANT OF BOVINE INSULIN''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1HO0 is a [[Single protein]] structure | + | 1HO0 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HO0 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Prigent, Y.]] | [[Category: Prigent, Y.]] | ||
[[Category: Richard, T.]] | [[Category: Richard, T.]] | ||
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Revision as of 16:03, 2 May 2008
NEW B-CHAIN MUTANT OF BOVINE INSULIN
Overview
The solution structure of a new B-chain mutant of bovine insulin, in which the cysteines B7 and B19 are replaced by two serines, has been determined by circular dichroism, 2D-NMR and molecular modeling. This structure is compared with that of the oxidized B-chain of bovine insulin [Hawkins et al. (1995) Int. J. Peptide Protein Res.46, 424-433]. Circular dichroism spectroscopy showed in particular that a higher percentage of helical secondary structure for the B-chain mutant is estimated in trifluoroethanol solution in comparison with the oxidized B-chain. 2D-NMR experiments confirmed, among multiple conformations, that the B-chain mutant presents defined secondary structures such as a alpha-helix between residues B9 and B19, and a beta-turn between amino acids B20 and B23 in aqueous trifluoroethanol. The 3D structures, which are consistent with NMR data and were obtained using a simulated annealing protocol, showed that the tertiary structure of the B-chain mutant is better resolved and is more in agreement with the insulin crystal structure than the oxidized B-chain structure described by Hawkins et al. An explanation could be the presence of two sulfonate groups in the oxidized insulin B-chain. Either by their charges and/or their size, such chemical groups could play a destructuring effect and thus could favor peptide flexibility and conformational averaging. Thus, this study provides new insights on the folding of isolated B-chains.
About this Structure
1HO0 is a Single protein structure. Full crystallographic information is available from OCA.
Reference
A new B-chain mutant of insulin: comparison with the insulin crystal structure and role of sulfonate groups in the B-chain structure., Dupradeau FY, Richard T, Le Flem G, Oulyadi H, Prigent Y, Monti JP, J Pept Res. 2002 Jul;60(1):56-64. PMID:12081626 Page seeded by OCA on Fri May 2 19:03:20 2008
