6zc5

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
==Human Adenovirus serotype D10 FiberKnob protein==
==Human Adenovirus serotype D10 FiberKnob protein==
-
<StructureSection load='6zc5' size='340' side='right'caption='[[6zc5]]' scene=''>
+
<StructureSection load='6zc5' size='340' side='right'caption='[[6zc5]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZC5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZC5 FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[6zc5]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Adenovirus_type_10 Adenovirus type 10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZC5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZC5 FirstGlance]. <br>
-
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zc5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zc5 OCA], [https://pdbe.org/6zc5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zc5 RCSB], [https://www.ebi.ac.uk/pdbsum/6zc5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zc5 ProSAT]</span></td></tr>
+
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6stt|6stt]]</div></td></tr>
 +
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">L5 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28275 Adenovirus type 10])</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zc5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zc5 OCA], [https://pdbe.org/6zc5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zc5 RCSB], [https://www.ebi.ac.uk/pdbsum/6zc5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zc5 ProSAT]</span></td></tr>
</table>
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Oncolytic virotherapies (OV) hold immense clinical potential. OV based on human adenoviruses (HAdV) derived from HAdV with naturally low rates of pre-existing immunity will be beneficial for future clinical translation. We generated a low-seroprevalence HAdV-D10 serotype vector incorporating an alphavbeta6 integrin-selective peptide, A20, to target alphavbeta6-positive tumor cell types. HAdV-D10 has limited natural tropism. Structural and biological studies of HAdV-D10 knob protein highlighted low-affinity engagement with native adenoviral receptors CAR and sialic acid. HAdV-D10 fails to engage blood coagulation factor X, potentially eliminating "off-target" hepatic sequestration in vivo. We engineered an A20 peptide that selectively binds alphavbeta6 integrin into the DG loop of HAdV-D10 fiber knob. Assays in alphavbeta6+ cancer cell lines demonstrated significantly increased transduction mediated by alphavbeta6-targeted variants compared with controls, confirmed microscopically. HAdV-D10.A20 resisted neutralization by neutralizing HAdV-C5 sera. Systemic delivery of HAdV-D10.A20 resulted in significantly increased GFP expression in BT20 tumors. Replication-competent HAdV-D10.A20 demonstrated alphavbeta6 integrin-selective cell killing in vitro and in vivo. HAdV-D10 possesses characteristics of a promising virotherapy, combining low seroprevalence, weak receptor interactions, and reduced off-target uptake. Incorporation of an alphavbeta6 integrin-selective peptide resulted in HAdV-D10.A20, with significant potential for clinical translation.
 +
 +
Development of a low-seroprevalence, alphavbeta6 integrin-selective virotherapy based on human adenovirus type 10.,Bates EA, Davies JA, Vanova J, Nestic D, Meniel VS, Koushyar S, Cunliffe TG, Mundy RM, Moses E, Uusi-Kerttula HK, Baker AT, Cole DK, Majhen D, Rizkallah PJ, Phesse T, Chester JD, Parker AL Mol Ther Oncolytics. 2022 Mar 16;25:43-56. doi: 10.1016/j.omto.2022.03.007., eCollection 2022 Jun 16. PMID:35399606<ref>PMID:35399606</ref>
 +
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 6zc5" style="background-color:#fffaf0;"></div>
 +
== References ==
 +
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
 +
[[Category: Adenovirus type 10]]
[[Category: Large Structures]]
[[Category: Large Structures]]
-
[[Category: Baker AT]]
+
[[Category: Baker, A T]]
-
[[Category: Mundy RM]]
+
[[Category: Mundy, R M]]
-
[[Category: Parker AL]]
+
[[Category: Parker, A L]]
-
[[Category: Rizkallah PJ]]
+
[[Category: Rizkallah, P J]]
 +
[[Category: Ad10]]
 +
[[Category: Adenoviridae]]
 +
[[Category: Adenovirus]]
 +
[[Category: Adv]]
 +
[[Category: Fiber]]
 +
[[Category: Fiber-knob]]
 +
[[Category: Fibre]]
 +
[[Category: Hadv]]
 +
[[Category: Hadv-d10]]
 +
[[Category: Head]]
 +
[[Category: Knob domain]]
 +
[[Category: Piv]]
 +
[[Category: Protein iv]]
 +
[[Category: Serotype 10]]
 +
[[Category: Viral protein]]

Revision as of 05:22, 13 July 2022

Human Adenovirus serotype D10 FiberKnob protein

PDB ID 6zc5

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6zc5"
Personal tools