7qd0
From Proteopedia
(Difference between revisions)
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==Structure of the orange carotenoid protein from Planktothrix agardhii binding echinenone in the C2 space group== | ==Structure of the orange carotenoid protein from Planktothrix agardhii binding echinenone in the C2 space group== | ||
| - | <StructureSection load='7qd0' size='340' side='right'caption='[[7qd0]]' scene=''> | + | <StructureSection load='7qd0' size='340' side='right'caption='[[7qd0]], [[Resolution|resolution]] 1.70Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QD0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QD0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7qd0]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QD0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QD0 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qd0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qd0 OCA], [https://pdbe.org/7qd0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qd0 RCSB], [https://www.ebi.ac.uk/pdbsum/7qd0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qd0 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=ECH:BETA,BETA-CAROTEN-4-ONE'>ECH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qd0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qd0 OCA], [https://pdbe.org/7qd0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qd0 RCSB], [https://www.ebi.ac.uk/pdbsum/7qd0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qd0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The orange carotenoid protein (OCP) is a photoactive protein involved in cyanobacterial photoprotection. Here, we report on the functional, spectral and structural characteristics of the peculiar Planktothrix PCC7805 OCP (Plankto-OCP). We show that this OCP variant is characterized by higher photoactivation and recovery rates, and a stronger energy-quenching activity, compared to other OCP studied thus far. We characterize the effect of the functionalizing carotenoid and of his-tagging on these reactions, and identify the time scales on which these modifications affect photoactivation. The presence of a his-tag at the C-terminus has a large influence on photoactivation, thermal recovery and PBS-fluorescence quenching, and likewise for the nature of the carotenoid that additionally affects the yield and characteristics of excited states and the ns-s dynamics of photoactivated OCP. By solving the structures of Plankto-OCP in the ECN- and CAN-functionalized states, each in two closely-related crystal forms, we further unveil the molecular breathing motions that animate Plankto-OCP at the monomer and dimer levels. We finally discuss the structural changes that could explain the peculiar properties of Plankto-OCP. | ||
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| + | Structure-function-dynamics relationships in the peculiar Planktothrix PCC7805 OCP1: Impact of his-tagging and carotenoid type.,Wilson A, Andreeva EA, Nizinski SJ, Talbot L, Hartmann E, Schlichting I, Burdzinski G, Sliwa M, Kirilovsky D, Colletier JP Biochim Biophys Acta Bioenerg. 2022 Jun 23;1863(7):148584. doi:, 10.1016/j.bbabio.2022.148584. PMID:35752265<ref>PMID:35752265</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7qd0" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Andreeva | + | [[Category: Andreeva, E A]] |
| - | [[Category: Colletier J | + | [[Category: Colletier, J P]] |
| - | [[Category: Hartmann E]] | + | [[Category: Hartmann, E]] |
| - | [[Category: Schlichting I]] | + | [[Category: Schlichting, I]] |
| + | [[Category: Carotenoid binding protein]] | ||
| + | [[Category: Carotenoid-binding]] | ||
| + | [[Category: Photoprotection]] | ||
| + | [[Category: Photosynthesis]] | ||
Revision as of 05:25, 13 July 2022
Structure of the orange carotenoid protein from Planktothrix agardhii binding echinenone in the C2 space group
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