1hrh

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1hrh.gif|left|200px]]
[[Image:1hrh.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1hrh |SIZE=350|CAPTION= <scene name='initialview01'>1hrh</scene>, resolution 2.4&Aring;
+
The line below this paragraph, containing "STRUCTURE_1hrh", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND=
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY=
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1hrh| PDB=1hrh | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hrh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hrh OCA], [http://www.ebi.ac.uk/pdbsum/1hrh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hrh RCSB]</span>
+
-
}}
+
'''CRYSTAL STRUCTURE OF THE RIBONUCLEASE H DOMAIN OF HIV-1 REVERSE TRANSCRIPTASE'''
'''CRYSTAL STRUCTURE OF THE RIBONUCLEASE H DOMAIN OF HIV-1 REVERSE TRANSCRIPTASE'''
Line 27: Line 24:
[[Category: /II, J F.Davies.]]
[[Category: /II, J F.Davies.]]
[[Category: Matthews, D A.]]
[[Category: Matthews, D A.]]
-
[[Category: hydrolase(endoribonuclease)]]
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:09:39 2008''
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:09:27 2008''
+

Revision as of 16:09, 2 May 2008

Image:1hrh.gif

Template:STRUCTURE 1hrh

CRYSTAL STRUCTURE OF THE RIBONUCLEASE H DOMAIN OF HIV-1 REVERSE TRANSCRIPTASE


Overview

The crystal structure of the ribonuclease (RNase) H domain of HIV-1 reverse transcriptase (RT) has been determined at a resolution of 2.4 A and refined to a crystallographic R factor of 0.20. The protein folds into a five-stranded mixed beta sheet flanked by an asymmetric distribution of four alpha helices. Two divalent metal cations bind in the active site surrounded by a cluster of four conserved acidic amino acid residues. The overall structure is similar in most respects to the RNase H from Escherichia coli. Structural features characteristic of the retroviral protein suggest how it may interface with the DNA polymerase domain of p66 in the mature RT heterodimer. These features also offer insights into why the isolated RNase H domain is catalytically inactive but when combined in vitro with the isolated p51 domain of RT RNase H activity can be reconstituted. Surprisingly, the peptide bond cleaved by HIV-1 protease near the polymerase-RNase H junction of p66 is completely inaccessible to solvent in the structure reported here. This suggests that the homodimeric p66-p66 precursor of mature RT is asymmetric with one of the two RNase H domains at least partially unfolded.

About this Structure

1HRH is a Single protein structure of sequence from Human immunodeficiency virus 1. Full crystallographic information is available from OCA.

Reference

Crystal structure of the ribonuclease H domain of HIV-1 reverse transcriptase., Davies JF 2nd, Hostomska Z, Hostomsky Z, Jordan SR, Matthews DA, Science. 1991 Apr 5;252(5002):88-95. PMID:1707186 Page seeded by OCA on Fri May 2 19:09:39 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1hrh"
Personal tools