This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

3vv5

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 19:05, 27 July 2022

Crystal structure of TTC0807 complexed with (S)-2-aminoethyl-L-cysteine (AEC)

Structural highlights

3vv5 is a 2 chain structure with sequence from Thet2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	3vvf, 3vve, 3vvd
Gene:	TTC0807, TT_C0807 (THET2)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Thermus thermophilus exhibits hypersensitivity to a lysine analog, (S)-2-aminoethyl-cysteine (AEC). Cosmid libraries were constructed using genomes from two AEC-resistant mutants, AT10 and AT14, and the cosmids that conferred AEC resistance on the wild-type strain were isolated. When the cosmid library for mutant AT14 was screened, two independent cosmids, conferring partial AEC resistance to the wild type, were obtained. Two cosmids carried a common genomic region from TTC0795 to TTC0810. This region contains genes encoding an ATP-binding cassette (ABC) transporter consisting of TTC0806/TTC0795, using TTC0807 as the periplasmic substrate-binding protein. Sequencing revealed that AT14 carries mutations in TTC0795 and TTC0969, causing decreases in the thermostability of the products. TTC0969 encodes the nucleotide-binding protein of a different ABC transporter consisting of TTC0967/TTC0968/TTC0969/TTC0970 using TTC0966 as the periplasmic substrate-binding protein. By similar screening for cosmids constructed for the mutant AT10, mutations were found at TTC0807 and TTC0969. Mutation in either of the transporter components gave partial resistance to AEC in the wild-type strain, while mutations of both transporters conferred complete AEC resistance. This result indicates that both transporters are involved in AEC uptake in T. thermophilus. To elucidate the mechanism of AEC uptake, crystal structures of TTC0807 were determined in several substrate-binding forms. The structures revealed that TTC0807 recognizes various basic amino acids by changing the side-chain conformation of Glu19, which interacts with the side-chain amino groups of the substrates.

Two ATP-Binding Cassette Transporters Involved in (S)-2-Aminoethyl-Cysteine Uptake in Thermus thermophilus.,Kanemaru Y, Hasebe F, Tomita T, Kuzuyama T, Nishiyama M J Bacteriol. 2013 Sep;195(17):3845-53. doi: 10.1128/JB.00202-13. Epub 2013 Jun, 21. PMID:23794618^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kanemaru Y, Hasebe F, Tomita T, Kuzuyama T, Nishiyama M. Two ATP-Binding Cassette Transporters Involved in (S)-2-Aminoethyl-Cysteine Uptake in Thermus thermophilus. J Bacteriol. 2013 Sep;195(17):3845-53. doi: 10.1128/JB.00202-13. Epub 2013 Jun, 21. PMID:23794618 doi:10.1128/JB.00202-13

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3vv5"

@@ Line 1: / Line 1: @@
 ==Crystal structure of TTC0807 complexed with (S)-2-aminoethyl-L-cysteine (AEC)==
-<StructureSection load='3vv5' size='340' side='right' caption='[[3vv5]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+<StructureSection load='3vv5' size='340' side='right'caption='[[3vv5]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3vv5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet2 Thet2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VV5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VV5 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3vv5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thet2 Thet2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VV5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VV5 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SLZ:L-THIALYSINE'>SLZ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SLZ:L-THIALYSINE'>SLZ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3vvf|3vvf]], [[3vve|3vve]], [[3vvd|3vvd]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3vvf|3vvf]], [[3vve|3vve]], [[3vvd|3vvd]]</div></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TTC0807, TT_C0807 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=262724 THET2])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TTC0807, TT_C0807 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=262724 THET2])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vv5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vv5 OCA], [http://pdbe.org/3vv5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vv5 RCSB], [http://www.ebi.ac.uk/pdbsum/3vv5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vv5 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vv5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vv5 OCA], [https://pdbe.org/3vv5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vv5 RCSB], [https://www.ebi.ac.uk/pdbsum/3vv5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vv5 ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Thet2]]
 [[Category: Hasebe, F]]

3vv5

From Proteopedia

Revision as of 19:05, 27 July 2022

Crystal structure of TTC0807 complexed with (S)-2-aminoethyl-L-cysteine (AEC)

Structural highlights

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox