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| | ==Crystal structure of Cex1p== | | ==Crystal structure of Cex1p== |
| - | <StructureSection load='3vwa' size='340' side='right' caption='[[3vwa]], [[Resolution|resolution]] 2.20Å' scene=''> | + | <StructureSection load='3vwa' size='340' side='right'caption='[[3vwa]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3vwa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VWA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VWA FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3vwa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VWA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VWA FirstGlance]. <br> |
| | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CEX1, O3240, YOR112W, YOR3240w ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr> | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CEX1, O3240, YOR112W, YOR3240w ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vwa OCA], [http://pdbe.org/3vwa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vwa RCSB], [http://www.ebi.ac.uk/pdbsum/3vwa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vwa ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vwa OCA], [https://pdbe.org/3vwa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vwa RCSB], [https://www.ebi.ac.uk/pdbsum/3vwa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vwa ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CEX1_YEAST CEX1_YEAST]] Component of the nuclear tRNA export machinery that my collect tRNA from the nuclear tRNA export receptors of the aminoacylation-dependent export and may deliver aminoacylated tRNAs to the translation machinery pathway at the nuclear pore complex.<ref>PMID:17203074</ref> | + | [[https://www.uniprot.org/uniprot/CEX1_YEAST CEX1_YEAST]] Component of the nuclear tRNA export machinery that my collect tRNA from the nuclear tRNA export receptors of the aminoacylation-dependent export and may deliver aminoacylated tRNAs to the translation machinery pathway at the nuclear pore complex.<ref>PMID:17203074</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Baker's yeast]] | | [[Category: Baker's yeast]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Ishitani, R]] | | [[Category: Ishitani, R]] |
| | [[Category: Nozawa, K]] | | [[Category: Nozawa, K]] |
| Structural highlights
Function
[CEX1_YEAST] Component of the nuclear tRNA export machinery that my collect tRNA from the nuclear tRNA export receptors of the aminoacylation-dependent export and may deliver aminoacylated tRNAs to the translation machinery pathway at the nuclear pore complex.[1]
Publication Abstract from PubMed
In all eukaryotes, transcribed precursor tRNAs are maturated by processing and modification processes in nucleus and are transported to the cytoplasm. The cytoplasmic export protein (Cex1p) captures mature tRNAs from the nuclear export receptor (Los1p) on the cytoplasmic side of the nuclear pore complex, and it delivers them to eukaryotic elongation factor 1alpha. This conserved Cex1p function is essential for the quality control of mature tRNAs to ensure accurate translation. However, the structural basis of how Cex1p recognizes tRNAs and shuttles them to the translational apparatus remains unclear. Here, we solved the 2.2 A resolution crystal structure of Saccharomyces cerevisiae Cex1p with C-terminal 197 disordered residues truncated. Cex1p adopts an elongated architecture, consisting of N-terminal kinase-like and a C-terminal alpha-helical HEAT repeat domains. Structure-based biochemical analyses suggested that Cex1p binds tRNAs on its inner side, using the positively charged HEAT repeat surface and the C-terminal disordered region. The N-terminal kinase-like domain acts as a scaffold to interact with the Ran-exportin (Los1p.Gsp1p) machinery. These results provide the structural basis of Los1p.Gsp1p.Cex1p.tRNA complex formation, thus clarifying the dynamic mechanism of tRNA shuttling from exportin to the translational apparatus.
Crystal structure of Cex1p reveals the mechanism of tRNA trafficking between nucleus and cytoplasm.,Nozawa K, Ishitani R, Yoshihisa T, Sato M, Arisaka F, Kanamaru S, Dohmae N, Mangroo D, Senger B, Becker HD, Nureki O Nucleic Acids Res. 2013 Apr 1;41(6):3901-14. doi: 10.1093/nar/gkt010. Epub 2013, Feb 8. PMID:23396276[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ McGuire AT, Mangroo D. Cex1p is a novel cytoplasmic component of the Saccharomyces cerevisiae nuclear tRNA export machinery. EMBO J. 2007 Jan 24;26(2):288-300. Epub 2007 Jan 4. PMID:17203074 doi:10.1038/sj.emboj.7601493
- ↑ Nozawa K, Ishitani R, Yoshihisa T, Sato M, Arisaka F, Kanamaru S, Dohmae N, Mangroo D, Senger B, Becker HD, Nureki O. Crystal structure of Cex1p reveals the mechanism of tRNA trafficking between nucleus and cytoplasm. Nucleic Acids Res. 2013 Apr 1;41(6):3901-14. doi: 10.1093/nar/gkt010. Epub 2013, Feb 8. PMID:23396276 doi:10.1093/nar/gkt010
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