7dwc
From Proteopedia
(Difference between revisions)
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==Bacteroides thetaiotaomicron VPI5482 BTAxe1== | ==Bacteroides thetaiotaomicron VPI5482 BTAxe1== | ||
| - | <StructureSection load='7dwc' size='340' side='right'caption='[[7dwc]]' scene=''> | + | <StructureSection load='7dwc' size='340' side='right'caption='[[7dwc]], [[Resolution|resolution]] 1.80Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DWC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DWC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7dwc]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DWC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DWC FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dwc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dwc OCA], [https://pdbe.org/7dwc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dwc RCSB], [https://www.ebi.ac.uk/pdbsum/7dwc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dwc ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dwc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dwc OCA], [https://pdbe.org/7dwc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dwc RCSB], [https://www.ebi.ac.uk/pdbsum/7dwc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dwc ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Gut bacteria-derived enzymes play important roles in the metabolism of dietary fiber through enabling the hydrolysis of polysaccharides. In this study, we identified and characterized a 29 kDa novel acetyl xylan esterase, BTAxe1, from Bacteroides thetaiotaomicron VPI5482. Then, we solved the structure of BTAxe1 and performed the rational design. Mutants N65S and N65A increased the activities toward short-chain (pNPA, pNPB) to near four-fold, and gained the activities toward longer-chain substrate (pNPO). Molecular docking analysis showed that the mutant N65S had a larger substrate binding pocket than the wild type. Hydrolysis studies using natural substrates showed that either N65S or N65A showed higher activity of that of wild-type, yielding 131.31 and 136.09 mM of acetic acid from xylan. This is the first study on the rational design of gut bacteria-derived Axes with broadened substrate specificity and enhanced activity, which can be referenced by other acetyl esterases or gut-derived enzymes. | ||
| + | |||
| + | Rational Design for Broadened Substrate Specificity and Enhanced Activity of a Novel Acetyl Xylan Esterase from Bacteroides thetaiotaomicron.,Wang L, Han X, Wang Y, Wei X, Liu S, Shao S, Yang S, Sun L, Xin F J Agric Food Chem. 2021 Jun 16;69(23):6665-6675. doi: 10.1021/acs.jafc.1c00750., Epub 2021 Jun 2. PMID:34074097<ref>PMID:34074097</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7dwc" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Sun | + | [[Category: Sun, L C]] |
| - | [[Category: Wang | + | [[Category: Wang, L Y]] |
| - | [[Category: Wang | + | [[Category: Wang, Y L]] |
| - | [[Category: Xin | + | [[Category: Xin, F J]] |
| + | [[Category: Acetyl xylan esterase]] | ||
| + | [[Category: Bacteroides thetaiotaomicron vpi5482]] | ||
| + | [[Category: Gut bacteria-derived]] | ||
| + | [[Category: Hydrolase]] | ||
Revision as of 04:58, 3 August 2022
Bacteroides thetaiotaomicron VPI5482 BTAxe1
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