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7wkq

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==Crystal Structure of halohydrin dehalogenase from Acidimicrobiia bacterium==
==Crystal Structure of halohydrin dehalogenase from Acidimicrobiia bacterium==
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<StructureSection load='7wkq' size='340' side='right'caption='[[7wkq]]' scene=''>
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<StructureSection load='7wkq' size='340' side='right'caption='[[7wkq]], [[Resolution|resolution]] 2.89&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WKQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WKQ FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7wkq]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WKQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WKQ FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wkq OCA], [https://pdbe.org/7wkq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wkq RCSB], [https://www.ebi.ac.uk/pdbsum/7wkq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wkq ProSAT]</span></td></tr>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wkq OCA], [https://pdbe.org/7wkq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wkq RCSB], [https://www.ebi.ac.uk/pdbsum/7wkq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wkq ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We report the discovery of an unusual halohydrin dehalogenase, HHDHamb, that can work under relative low acidic conditions and extremely low temperatures for the bio-nitration of epoxides using nitrite as a nitrating agent. The bio-nitration strategy exhibits high chemo-, regio-, and enantioselectivity, catalyzing the kinetic resolution of various epoxides to enantiopure beta -nitroalcohols with nitro-bearing stereocenters in up to 41% isolated yield and &gt;99% enantiomeric excess ( ee ). Additionally, the bio-nitration method displays a high reaction efficiency and can be performed on a gram scale. We also solved the crystal structure of HHDHamb to understand the possible structural determinants of chemoselectivity control in the bio-nitration reaction.
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Identification and Structure Analysis of an Unusual Halohydrin Dehalogenase for Highly Chemo-, Regio- and Enantioselective Bio-Nitration of Epoxides.,Wang HH, Wan NW, Miao RP, He CL, Chen YZ, Liu ZQ, Zheng YG Angew Chem Int Ed Engl. 2022 Jul 20. doi: 10.1002/anie.202205790. PMID:35856897<ref>PMID:35856897</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7wkq" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Wan NW]]
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[[Category: Wan, N W]]
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[[Category: Biosynthetic protein]]
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[[Category: Halohydrin dehalogenase]]

Revision as of 05:05, 3 August 2022

Crystal Structure of halohydrin dehalogenase from Acidimicrobiia bacterium

PDB ID 7wkq

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