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| | ==Crystal Structure of the Sld3/Treslin domain from yeast Sld3== | | ==Crystal Structure of the Sld3/Treslin domain from yeast Sld3== |
| - | <StructureSection load='3wi3' size='340' side='right' caption='[[3wi3]], [[Resolution|resolution]] 2.40Å' scene=''> | + | <StructureSection load='3wi3' size='340' side='right'caption='[[3wi3]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3wi3]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WI3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WI3 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3wi3]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WI3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WI3 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SLD3, YGL113W, G2980 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr> | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SLD3, YGL113W, G2980 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wi3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wi3 OCA], [http://pdbe.org/3wi3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wi3 RCSB], [http://www.ebi.ac.uk/pdbsum/3wi3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wi3 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wi3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wi3 OCA], [https://pdbe.org/3wi3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wi3 RCSB], [https://www.ebi.ac.uk/pdbsum/3wi3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wi3 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SLD3_YEAST SLD3_YEAST]] Required for loading and maintenance of stable association of CDC45 with chromatin during initiation and elongation of DNA replication. Also involved in temporal regulation of origin firing. Required for the association of PSF1 with origins.<ref>PMID:11296242</ref> <ref>PMID:12730134</ref> <ref>PMID:21487389</ref> | + | [[https://www.uniprot.org/uniprot/SLD3_YEAST SLD3_YEAST]] Required for loading and maintenance of stable association of CDC45 with chromatin during initiation and elongation of DNA replication. Also involved in temporal regulation of origin firing. Required for the association of PSF1 with origins.<ref>PMID:11296242</ref> <ref>PMID:12730134</ref> <ref>PMID:21487389</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Baker's yeast]] | | [[Category: Baker's yeast]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Araki, H]] | | [[Category: Araki, H]] |
| | [[Category: Itou, H]] | | [[Category: Itou, H]] |
| Structural highlights
Function
[SLD3_YEAST] Required for loading and maintenance of stable association of CDC45 with chromatin during initiation and elongation of DNA replication. Also involved in temporal regulation of origin firing. Required for the association of PSF1 with origins.[1] [2] [3]
Publication Abstract from PubMed
The initiation of eukaryotic chromosomal DNA replication requires the formation of an active replicative helicase at the replication origins of chromosomal DNA. Yeast Sld3 and its metazoan counterpart Treslin are the hub proteins mediating protein associations critical for the helicase formation. Here, we show the crystal structure of the central domain of Sld3 that is conserved in Sld3/Treslin family of proteins. The domain consists of two segments with 12 helices and is sufficient to bind to Cdc45, the essential helicase component. The structure model of the Sld3-Cdc45 complex, which is crucial for the formation of the active helicase, is proposed.
Crystal structure of the homology domain of the eukaryotic DNA replication proteins sld3/treslin.,Itou H, Muramatsu S, Shirakihara Y, Araki H Structure. 2014 Sep 2;22(9):1341-7. doi: 10.1016/j.str.2014.07.001. Epub 2014 Aug, 7. PMID:25126958[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kamimura Y, Tak YS, Sugino A, Araki H. Sld3, which interacts with Cdc45 (Sld4), functions for chromosomal DNA replication in Saccharomyces cerevisiae. EMBO J. 2001 Apr 17;20(8):2097-107. PMID:11296242 doi:http://dx.doi.org/10.1093/emboj/20.8.2097
- ↑ Takayama Y, Kamimura Y, Okawa M, Muramatsu S, Sugino A, Araki H. GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast. Genes Dev. 2003 May 1;17(9):1153-65. PMID:12730134 doi:http://dx.doi.org/10.1101/gad.1065903
- ↑ Tanaka T, Umemori T, Endo S, Muramatsu S, Kanemaki M, Kamimura Y, Obuse C, Araki H. Sld7, an Sld3-associated protein required for efficient chromosomal DNA replication in budding yeast. EMBO J. 2011 May 18;30(10):2019-30. doi: 10.1038/emboj.2011.115. Epub 2011 Apr, 12. PMID:21487389 doi:http://dx.doi.org/10.1038/emboj.2011.115
- ↑ Itou H, Muramatsu S, Shirakihara Y, Araki H. Crystal structure of the homology domain of the eukaryotic DNA replication proteins sld3/treslin. Structure. 2014 Sep 2;22(9):1341-7. doi: 10.1016/j.str.2014.07.001. Epub 2014 Aug, 7. PMID:25126958 doi:http://dx.doi.org/10.1016/j.str.2014.07.001
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