1hv6

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[[Image:1hv6.gif|left|200px]]
[[Image:1hv6.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1hv6 |SIZE=350|CAPTION= <scene name='initialview01'>1hv6</scene>, resolution 2.00&Aring;
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The line below this paragraph, containing "STRUCTURE_1hv6", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=GCU:D-GLUCURONIC+ACID'>GCU</scene>, <scene name='pdbligand=MAV:D-MANNURONIC+ACID'>MAV</scene>, <scene name='pdbligand=MAW:4-DEOXY-D-MANNURONIC+ACID'>MAW</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Poly(beta-D-mannuronate)_lyase Poly(beta-D-mannuronate) lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.3 4.2.2.3] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1hv6| PDB=1hv6 | SCENE= }}
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|RELATEDENTRY=[[1qaz|1QAZ]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hv6 OCA], [http://www.ebi.ac.uk/pdbsum/1hv6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hv6 RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF ALGINATE LYASE A1-III COMPLEXED WITH TRISACCHARIDE PRODUCT.'''
'''CRYSTAL STRUCTURE OF ALGINATE LYASE A1-III COMPLEXED WITH TRISACCHARIDE PRODUCT.'''
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==Reference==
==Reference==
Crystal structure of alginate lyase A1-III complexed with trisaccharide product at 2.0 A resolution., Yoon HJ, Hashimoto W, Miyake O, Murata K, Mikami B, J Mol Biol. 2001 Mar 16;307(1):9-16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11243798 11243798]
Crystal structure of alginate lyase A1-III complexed with trisaccharide product at 2.0 A resolution., Yoon HJ, Hashimoto W, Miyake O, Murata K, Mikami B, J Mol Biol. 2001 Mar 16;307(1):9-16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11243798 11243798]
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[[Category: Poly(beta-D-mannuronate) lyase]]
 
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Sphingomonas species]]
[[Category: Sphingomonas species]]
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[[Category: Murata, K.]]
[[Category: Murata, K.]]
[[Category: Yoon, H J.]]
[[Category: Yoon, H J.]]
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[[Category: alginate lyase]]
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[[Category: Alginate lyase]]
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[[Category: trisaccharide complex,alpha barrel]]
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[[Category: Trisaccharide complex,alpha barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:15:50 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:10:50 2008''
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Revision as of 16:15, 2 May 2008

Image:1hv6.gif

Template:STRUCTURE 1hv6

CRYSTAL STRUCTURE OF ALGINATE LYASE A1-III COMPLEXED WITH TRISACCHARIDE PRODUCT.


Overview

The structure of A1-III from a Sphingomonas species A1 complexed with a trisaccharide product (4-deoxy-l-erythro-hex-4-enepyranosyluronate-mannuronate-mannuronic acid) was determined by X-ray crystallography at 2.0 A with an R-factor of 0.16. The final model of the complex form comprising 351 amino acid residues, 245 water molecules, one sulfate ion and one trisaccharide product exhibited a C(alpha) r.m.s.d. value of 0.154 A with the reported apo form of the enzyme. The trisaccharide was bound in the active cleft at subsites -3 approximately -1 from the non-reducing end by forming several hydrogen bonds and van der Waals interactions with protein atoms. The catalytic residue was estimated to be Tyr246, which existed between subsites -1 and +1 based on a mannuronic acid model oriented at subsite +1.

About this Structure

1HV6 is a Single protein structure of sequence from Sphingomonas species. Full crystallographic information is available from OCA.

Reference

Crystal structure of alginate lyase A1-III complexed with trisaccharide product at 2.0 A resolution., Yoon HJ, Hashimoto W, Miyake O, Murata K, Mikami B, J Mol Biol. 2001 Mar 16;307(1):9-16. PMID:11243798 Page seeded by OCA on Fri May 2 19:15:50 2008

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