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spinqualitylabelsall models 1fze, resolution 3.0Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

CRYSTAL STRUCTURE OF FRAGMENT DOUBLE-D FROM HUMAN FIBRIN

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

The structure of fragment double-D from human fibrin has been solved in, the presence and absence of the peptide ligands that simulate the two, knobs exposed by the removal of fibrinopeptides A and B, respectively. All, told, six crystal structures have been determined, three of which are, reported here for the first time: namely, fragments D and double-D with, the peptide GHRPam alone and double-D in the absence of any peptide, ligand. Comparison of the structures has revealed a series of, conformational changes that are brought about by the various knob-hole, interactions. Of greatest interest is a moveable "flap" of two negatively, charged amino acids (Glubeta397 and Aspbeta398) whose side chains are, pinned back to the coiled coil with a calcium atom bridge until GHRPam, occupies the beta-chain pocket. Additionally, in the absence of the, peptide ligand GPRPam, GHRPam binds to the gamma-chain pocket, a new, calcium-binding site being formed concomitantly.

Disease

Known diseases associated with this structure: Afibrinogenemia, congenital OMIM:[134820], Afibrinogenemia, congenital OMIM:[134830], Amyloidosis, hereditary renal OMIM:[134820], Dysfibrinogenemia, alpha type, causing bleeding diathesis OMIM:[134820], Dysfibrinogenemia, alpha type, causing recurrent thrombosis OMIM:[134820], Dysfibrinogenemia, beta type OMIM:[134830], Dysfibrinogenemia, gamma type OMIM:[134850], Hypofibrinogenemia, gamma type OMIM:[134850], Thrombophilia, dysfibrinogenemic OMIM:[134830], Thrombophilia, dysfibrinogenemic OMIM:[134850]

About this Structure

1FZE is a Protein complex structure of sequences from Homo sapiens with NAG and CA as ligands. Full crystallographic information is available from OCA.

Reference

Conformational changes in fragments D and double-D from human fibrin(ogen) upon binding the peptide ligand Gly-His-Arg-Pro-amide., Everse SJ, Spraggon G, Veerapandian L, Doolittle RF, Biochemistry. 1999 Mar 9;38(10):2941-6. PMID:10074346

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