1hwp
From Proteopedia
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[[Image:1hwp.gif|left|200px]] | [[Image:1hwp.gif|left|200px]] | ||
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| - | | | + | {{STRUCTURE_1hwp| PDB=1hwp | SCENE= }} |
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'''EBULIN COMPLEXED WITH PTEROIC ACID, TRIGONAL CRYSTAL FORM''' | '''EBULIN COMPLEXED WITH PTEROIC ACID, TRIGONAL CRYSTAL FORM''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWP OCA]. | |
==Reference== | ==Reference== | ||
2.8-A crystal structure of a nontoxic type-II ribosome-inactivating protein, ebulin l., Pascal JM, Day PJ, Monzingo AF, Ernst SR, Robertus JD, Iglesias R, Perez Y, Ferreras JM, Citores L, Girbes T, Proteins. 2001 May 15;43(3):319-26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11288182 11288182] | 2.8-A crystal structure of a nontoxic type-II ribosome-inactivating protein, ebulin l., Pascal JM, Day PJ, Monzingo AF, Ernst SR, Robertus JD, Iglesias R, Perez Y, Ferreras JM, Citores L, Girbes T, Proteins. 2001 May 15;43(3):319-26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11288182 11288182] | ||
| - | [[Category: | + | [[Category: RRNA N-glycosylase]] |
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[[Category: Day, P J.]] | [[Category: Day, P J.]] | ||
[[Category: Ernst, S R.]] | [[Category: Ernst, S R.]] | ||
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[[Category: Pascal, J M.]] | [[Category: Pascal, J M.]] | ||
[[Category: Robertus, J D.]] | [[Category: Robertus, J D.]] | ||
| - | [[Category: | + | [[Category: Inhibitor]] |
| - | [[Category: | + | [[Category: Ribosome-inactivating protein]] |
| - | [[Category: | + | [[Category: Ricin-like]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:18:12 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 16:18, 2 May 2008
EBULIN COMPLEXED WITH PTEROIC ACID, TRIGONAL CRYSTAL FORM
Overview
Ebulin l is a type-II ribosome-inactivating protein (RIP) isolated from the leaves of Sambucus ebulus L. As with other type-II RIP, ebulin is a disulfide-linked heterodimer composed of a toxic A chain and a galactoside-specific lectin B chain. A normal level of ribosome-inactivating N-glycosidase activity, characteristic of the A chain of type-II RIP, has been demonstrated for ebulin l. However, ebulin is considered a nontoxic type-II RIP due to a reduced cytotoxicity on whole cells and animals as compared with other toxic type-II RIP like ricin. The molecular cloning, amino acid sequence, and the crystal structure of ebulin l are presented and compared with ricin. Ebulin l is shown to bind an A-chain substrate analogue, pteroic acid, in the same manner as ricin. The galactoside-binding ability of ebulin l is demonstrated crystallographically with a complex of the B chain with galactose and with lactose. The negligible cytotoxicity of ebulin l is apparently due to a reduced affinity for galactosides. An altered mode of galactoside binding in the 2gamma subdomain of the lectin B chain primarily causes the reduced affinity.
About this Structure
Full crystallographic information is available from OCA.
Reference
2.8-A crystal structure of a nontoxic type-II ribosome-inactivating protein, ebulin l., Pascal JM, Day PJ, Monzingo AF, Ernst SR, Robertus JD, Iglesias R, Perez Y, Ferreras JM, Citores L, Girbes T, Proteins. 2001 May 15;43(3):319-26. PMID:11288182 Page seeded by OCA on Fri May 2 19:18:12 2008
