1hx2
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1hx2.jpg|left|200px]] | [[Image:1hx2.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1hx2", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_1hx2| PDB=1hx2 | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''SOLUTION STRUCTURE OF BSTI, A TRYPSIN INHIBITOR FROM BOMBINA BOMBINA.''' | '''SOLUTION STRUCTURE OF BSTI, A TRYPSIN INHIBITOR FROM BOMBINA BOMBINA.''' | ||
| Line 29: | Line 26: | ||
[[Category: Rosengren, K J.]] | [[Category: Rosengren, K J.]] | ||
[[Category: Scanlon, M J.]] | [[Category: Scanlon, M J.]] | ||
| - | [[Category: | + | [[Category: Beta-sheet disulfide-rich]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:18:56 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 16:18, 2 May 2008
SOLUTION STRUCTURE OF BSTI, A TRYPSIN INHIBITOR FROM BOMBINA BOMBINA.
Overview
The three-dimensional solution structure of BSTI, a trypsin inhibitor from the European frog Bombina bombina, has been solved using (1)H NMR spectroscopy. The 60 amino acid protein contains five disulfide bonds, which were unambiguously determined to be Cys (4--38), Cys (13--34), Cys (17--30), Cys (21--60), and Cys (40--54) by experimental restraints and subsequent structure calculations. The main elements of secondary structure are four beta-strands, arranged as two small antiparallel beta-sheets. The overall fold of BSTI is disk shaped and is characterized by the lack of a hydrophobic core. The presumed active site is located on a loop comprising residues 21--34, which is a relatively disordered region similar to that seen in many other protease inhibitors. However, the overall fold is different to other known protease inhibitors with the exception of a small family of inhibitors isolated from nematodes of the family Ascaris and recently also from the haemolymph of Apis mellifera. BSTI may thus be classified as a new member of this recently discovered family of protease inhibitors.
About this Structure
1HX2 is a Single protein structure of sequence from Bombina bombina. Full crystallographic information is available from OCA.
Reference
Solution structure of BSTI: a new trypsin inhibitor from skin secretions of Bombina bombina., Rosengren KJ, Daly NL, Scanlon MJ, Craik DJ, Biochemistry. 2001 Apr 17;40(15):4601-9. PMID:11294627 Page seeded by OCA on Fri May 2 19:18:56 2008
